Abstract: In order to screen a bacteriocin-producing lactic acid bacteria and explore its bacteriocin gene cluster, the pathogensEscherichia coliandStaphylococcus aureuswere used as indicator, through initial and repeated screenings using conventional methods,Lactobacillus pentosusLpe59, a strain with strong antibacterial activity and bacteriocin production was isolated. The whole-genome information of strain Lpe59 was obtainde through high-throughput sequencing, the results showed the genome size of strain Lpe59 was 3274558 bp, encoding 3047 genes, with 2434, 2203, 1614, 107 and 4 functional genes annotated in COG, GO, KEGG, CAZY and VFDB databases, respectively. Using the online software AntiSMASH 6.11, four types of secondary metabolite gene clusters were predicted in strain Lpe59, including ribosomally synthesized and post-translationally modified peptide-like (RiPP-like) compounds, type Ⅲ polyketide synthase (T3PKS) metabolites, terpene, and cyclic-lactone-autoinducer. Additionally, a gene cluster for the synthesis of class Ⅱ bacteriocins Plantaricin K and Plantaricin EF was predicted through Bagel4. In conclusion, antibacterial tests and genomic predictions suggested thatL.pentosusLpe59 was a promising lactic acid bacterium with biocontrol potential and the ability to produce bacteriocins. The research findings provided a theoretical basis for the further development and application of its bacteriocins, Plantaricin K and Plantaricin EF.

Key words: Lactiplantibacillus pentosus, bacteriocin, bacteriostasis, whole genome, synthetic gene cluster