Abstract: To explore the impact of different cultivation periods on the exudates ofLavandula angustifoliain Yili, Xinjiang, non-targeted metabolomic analysis was conducted by using liquid chromatography-mass spectrometry (LC-MS) on the rhizosphere soil samples of 1-year-old, 3-year-old, and 5-year-old lavender plants(named L1, L3, and L5), as well as soil samples without lavender cultivation (L0). The results indicated that root exudates included nine main categories: organic acids, lipids, nucleic acids, carbohydrates, vitamins and cofactors, hormones, peptides, antibiotics, and steroids. Compared to L0, L1, L3, and L5 resulted in the identification of 26, 17, and 21 significantly different exudates, respectively (P＜0.001). Additionally, L1, L3, and L5 exhibited enrichment in 13, 10, and 9 significantly different metabolic pathways (P＜0.05), respectively. Compared to L1, L3 and L5 showed 27 (P＜0.05) and 9 significantly different exudates (P＜0.001), respectively, and enrichment in 4 and 9 significantly different metabolic pathways (P＜0.05), respectively. Furthermore, when compared to L3, L5 presented 13 significantly different exudates (P＜0.05) and enrichment in 6 significantly different metabolic pathways (P＜0.05). Overall, the exudates ofLavandula angustifoliaroot system underwent significant changes as the cultivation period extended, with sterols initially decreasing and then increasing, while vitamins, peptides, and nucleotides gradually decreased.

Key words: root exudate, untargeted metabolome, lavender, continuous planting, rhizosphere