生物学杂志

Journal of Biology ›› 2019, Vol. 36 ›› Issue (6): 47-.doi: 10.3969/j.issn.2095-1736.2019.06.047

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Effects of kisspeptin and N-carbamylgulamate on GnRH secretion in GT1-7 cells #br#

  

  1. 1. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097; 2. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070;3. College of Animal Science and Technology, China Agricultural University, Beijing 100193， China
  • Online:2019-12-18 Published:2019-12-12

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Abstract:

GT1-7 cell line is an ideal model to research GnRH secretion in vitro. In order to reveal the effects of kisspeptin and/or N-carbamylgulamate (NCG) on GnRH secretion, GT1-7 cells were used to evaluate the effect of kisspeptin and NCG alone or co-culture on cell proliferation and GnRH secretion. The results showed that either kisspeptin-10 or NCG could improve GT1-7 cell proliferation, co-culture with kisspeptin-10 and NCG presented a synergistic effect on cell number. Kisspeptin-10 challenge could stimulate GnRH secretion, but NCG showed an opposite effect. However, co-culture with kisspeptin-10 and NCG could compensate the decrease of GnRH secretion treated with NCG, under certain concentration of kisspeptin-10, NCG showed a dose-dependent increase of GnRH secretion. This work demonstrated the effects of kisspeptin and NCG on increasing GnRH secretion.


Key words: kisspeptin-10, N-carbamylgulamate, GT1-7 cells, gonadotropin-releasing hormone

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