Abstract: In the present work, a SH-SY5Y injuring model induced by Aβ1-40 was established, and the effect and possible mechanisms of Catechin on the model were investigated. Cell viability was assayed by MTT, and membrane permeability was detected by the LDH kit. In addition, the ROS kit and Caspase 3 activity assay kit were employed to measure the ROS level and Caspase 3 activity in SH-SY5Y cell, respectively. The results showed that, Aβ1-40 decreased the cell viability and promoted the membrane permeability of SH-SY5Y, but Catechin could inhibit the impact of Aβ1-40. The possible mechanism involved is that Catechin might decrease the Oxidation level and Caspase 3 activity in SH-SY5Y cell.

Key words: Catechin, Aβ1-40, SH-SY5Y