Abstract: A static bioassay test was used to study the cute toxicity of BPA(Bisphenol A) and phenol to the juvenile prawn of Macrobrachium nipponens, and based on which, toxic unit (TU) method was used to study the joint toxicity of BPA and phenol to Macrobrachium nipponense. The results showed that there were significant relationships between the death rate of the juvenile prawn and the concentrations of BPA and phenol. The LC50 decreased gradually with the increasing of the exposure time, and there were significant dose-effect and and time-effect. The 24 h LC50 and 48 h LC50 of the juvenile prawn to BPA were 25.68, 12.03 mg/L, respectively, and 33.63, 27.59 mg/L to phenol, respectively. The safe concentrations of the juvenile prawn to BPA and phenol were 0.80 and 5.57 mg/L, respectively. The LC50 of 24 h and 48 h joint toxicity of BPA and phenol to the juvenile prawn were 0.97 and 0.51 TU, respectively. The TUi was less than 1, which meant that the joint action type is synergistic.

Key words: bisphenol A, phenol, Macrobrachium nipponense, joint toxicity