Abstract: In order to design protein epitope polypeptide for important infectious virus of porcine, the candidate proteins vaccine of swine infection virus was selected. The selected proteins included GP5 protein of PRRSV (porcine reproductive and respiratory syndrome virus), CAP protein of PCV2 (porcine circovirus type 2), and E2 protein of CSFV (classical swine fever virus). ABCpred and BepiPred prediction programs were used to predict B cell epitopes, quantitative matrix, the artificial neural network was used to predict CTL cell epitopes, and an online server prediction was used to analysis MHC class Ⅱ peptide binding affinity. The secondary structure further verifying the accuracy of B/T cell epitopes was analyzed by SOPMA and DNASTAR software. The B/T cell epitopes were recomposed by protean program. The results showed that GP5 protein had 4 B cell epitopes, CAP and E2 had 5 B cell epitopes, and GP5, CAP and E2 protein had 1 CTL cell epitopes. GP5 and E2 protein had 2 Th cell epitopes, and CAP protein had 1 Th cell epitopes. The secondary structure showed that most of the B/T cell epitopes were located in the exposed surface, the random coil and the corner, which verified the prediction accuracy of B/T cell epitopes. The B/T cell epitopes were recomposed by protean program, and triple epitope vaccine of porcine virus holding better antigen was designed finally, and laid the foundation for the development of swine virus multi vaccine.

Key words: porcine virus, epitope vaccine, secondary structure analysis, antigenic analysis