Abstract: To construct miR-125a-3p overexpression and suppression lentivirus shRNA vectors for studying its effect to diarrhea of miR-125a-3p in mice,which laid a foundation of study miR-125a-3p to provide preliminary preparation for the impact of rotavirus proliferation process and to explore the molecular mechanism, the miR-125a-3p precursor sequence and miR-125a-3p reverse complement sequence were designed, and pLVshRNA-EGFP(2A)Puro vector was used to construct recombination lentivirus plasmid. The recombinant plasmid can generate the miR-125a-3p overexpression and suppression lentivirus vectors. Packaged lentivirus particle contained miR-125a-3p overexpression and suppression lentivirus by HEK293Ta. Virus titer was detected after infection of HEK293Ta. Finally we evaluated miR-125a-3p expression after that the virus infected MA104 cell and injected mice, and diarrhea of mice that was infected by rotavirus.It is successfully to construct the miRNA-125-3p overexpression and suppression lentivirus vectors. The recombinant virus successfully infected MA104 cells and mice small intestine. miR-125a-3p overexpression and suppression lentivirus vector were constructed successfully，which provide a stable cell transfection vector for further study of the impact and mechanism of miR-125a-3p to rotavirus and its host.We also found that the miR-125a-3p overexpression lentivirus vector can inhibitor the diarrhea,which was infected by rotavirus.

Key words: miR-125a-3p； lentivirus vector； overexpression vector； suppression vector, suckling mice