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Influences of magnetic immunochromatographic assay for detection of PreS1 region of hepatitis B virus surface antigen

  

  1. 1. Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation, Ministry of Agriculture, Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306； 2. Nanjing iNanoMax Medtech  Limited Company, Nanjing 211100；3. The Institute for Biomedical Engineering and Nano Science,Tongji University School of Medicine, Shanghai 200092；4. Department of Immunology,Tongji University School of Medicine, Shanghai 200092, China
  • Online:2017-02-18 Published:2017-02-18

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Abstract: To establish a rapid assay for HBV, a magnetic immunochromatographic assay (MICA) for detection of PreS1Ag in serum samples of people was developed in our study. The influences of hydrodynamic size of magnetic nanobeads, coupled antibody, pH, basal buffer, reaction temperature on the MICA were analyzed emphatically. Results showed that dispersion stability of magnetic nanobeads monitored by hydrodynamic size had a strong effect on MICA. Moreover, optimal antibody addition, proper pH value of 6.5 to 9 and using PBS with more stable pH as the basal buffer could be beneficial to improve the sensitivity and stability of MICA. The results of this research are expected to provide the theoretical guidance for the future development of MICA.

Key words: PreS1Ag, serum samples, hepatitis B test, immunochromatographic assay, magnetic nanobeads