Abstract: NewFlammulina velutipes-based composite material was prepared by freeze-drying method withFlammulina velutipesrich in chitin and dextran as raw material after extraction of polysaccharides. TheFlammulina velutipes-based composite was analyzed by infrared spectrum and scanning electron microscope. The samples characteristics such as anti-microbial properties, mechanical properties, swelling property, water vapor permeability, coagulation performancein vitro, degradability and cytotoxicity were assayed. Results showed thatFlammulina velutipessamples had excellent antibacterial properties, and the bacteriostatic rates ofEscherichia coliandStaphylococcus aureuswere 98.51% and 88.18% respectively.Flammulina velutipes-based composite dressing had excellent physical properties and high softness, and had excellent water absorption and water retention. Its breaking strength was 15.20 MPa and elongation was 84.11%. Its water vapor permeability number reached 2500 g/(m2·d), the coagulation indexin vitrowas 0.3. Moreover, the dressing was non-toxic and harmless, and the soil degradation rate could reach 79.79% after 35 days of discarding. This study demonstrated thatFlammulina velutipes-based composite dressing had excellent medical potential and could be used in wound dressing and tissue engineering. This study was of great significance to promote the utilization of by-products of fungal polysaccharide extraction and enhance the added value ofFlammulina velutipes.

Key words: fungal composite materials, medical dressing;Flammulina velutipes, chitin, gelatin