Abstract: The principle, application and common problems of peroxidase-based PL and biotin ligase-based PL are introduced. This review focuses on the current application of PL technique in the study of synaptic subregion molecular components in the field of neurobiology and its achievements, and analyzes the challenges faced in the application process, such as probe input and high background in living brain tissue. It is pointed out that this technique will provide an unprecedented opportunity to systematically identify the highly complex proteome of dynamic interaction and subcellular structure in neural tissue, and also provide an effective approach to search for key molecules in neurobiology.

Key words: proximity labeling, biotin ligase, peroxidase, biotin, neurobiology