Abstract: Soybean is an important nitrogen-fixing crop having strong amino acid metabolism and high activity of alanine aminotransferase during seed germination. Alanine aminotransferase can catalyze the reversible reaction of glutamic acid and pyruvate to produce alpha-ketoglutarate and alanine. This reaction is essential in the metabolism of amino acid in organisms. In this study, the possible sequence of Glycine max glutamic-alanine aminotransferase was obtained through database. We built a 3-D homology model of the protein sequence of Glycine max alanine aminotransferase (NP_001237567.2) with Discovery Studio, explored the conditions of the model in different concentrations of buffers with molecular dynamic, and obtained a stable model of solution state. The modification and optimization of the three-dimensional structure model of alanine aminotransferase prove the feasibility of adding histidine labelling to Glycine max alanine aminotransferase and make a theoretical preparation for the expression and separation of Glycine max alanine transaminase later.

Key words: glutamate-pyruvate transaminase, Glycine max, homologous modeling, poly-histidine tag