Abstract: tLipases has broad applications in food, paper, energy, biomedicine and other industrial fields, due to their advantages of high efficiency, environmental protection and substrate specificity. Most natural lipases fail to meet the actual industrial needs, such as low cost, strong organic solvent tolerance, temperature tolerance, therefore, the application of economic lipases for large-scale industrial production is still limited. The continuous progresses in genetic engineering and protein engineering technologies make it possible to modify and develop novel lipases suitable for industrial applications. In this paper, we reviewed the basic information of molecular structure and catalytic mechanism of lipases, and the recent progresses in lipase biological modification, especially the new technologies. The development trend of lipase biological modification was also prospected.

Key words: lipase, biological modification, directed evolution