生物学杂志

生物学杂志 ›› 2026, Vol. 43 ›› Issue (3): 67-.doi: 10.3969/j.issn.2095-1736.2026.03.067

• 研究报告 • 上一篇    下一篇

Pseudomonas hunanensisSK-4对含铜废水脱氮性能优化

张宇红， 蔡佳诚， 刘　健， 高雯嵩， 王溪若， 马学睿   

  1. 沈阳化工大学 环境与安全工程学院， 沈阳 110142
  • 出版日期:2026-06-18 发布日期:2026-06-16
  • 作者简介:张宇红，博士，副教授，主要研究水污染控制以及环境生物技术，E-mail：zhangyhhit@163.com
  • 基金资助:
    辽宁省科技厅应用基础研究计划项目（2023JH2/101300047）；辽宁省教育厅面上项目（LJKZ0471）

Optimization of nitrogen removal performance of Pseudomonas hunanensis SK-4 for copper-containing wastewater

ZHANG Yuhong, CAI Jiacheng, LIU Jian, GAO Wensong, WANG Xiruo, MA Xuerui   

  1. College of Environmental and Safety Engineering, Shenyang University of Chemical Technology, Shenyang 110142，China
  • Online:2026-06-18 Published:2026-06-16

摘要： 为解决生物脱氮系统容易受到含铜废水冲击，导致脱氮效率严重下降的问题，本研究结合Plackett-Burman试验、最陡爬坡试验和响应面试验三步法对Pseudomonas hunanensisSK-4的培养因素进行优化。Plackett-Burman试验结果表明，振荡速度、pH和接种量对菌株SK-4脱氮性能影响最为显著，且30~50 mg/L的Cu2+对菌株SK-4的脱氮效率影响并不明显，表明了菌株具有较强的Cu2+耐受能力。由响应面试验得到，在pH为7.83，接种量体积分数为3.95%、振荡速度为149.21 r/min的条件下，菌株对NH+4-N的最优去除率为82.33%。在实际废水小试实验中，在35.31 mg/L的Cu2+胁迫下，菌株SK-4依然能够高效降解污染物，对TN、NH+4-N、NO－3-N和COD的去除率分别达93.79%、98.52%、85.42%和89.90%。研究优化了菌株SK-4在含铜废水中的氮代谢效率，验证了菌株的实际应用潜力，为含铜废水的生物处理提供了新的处理思路。

关键词: 生物脱氮, 异养硝化—好氧反硝化, 响应面, 含铜废水, 工业水处理

Abstract: To address the issue that biological denitrification systems are prone to being impacted by copper-containing wastewater, leading to a significant decline in denitrification efficiency, this study optimized the culture factors ofPseudomonas hunanensisSK-4 through a three-step method combining the Plackett-Burman test, the steepest ascent test, and the response surface test. The results of the Plackett-Burman test indicated that the oscillation speed, pH, and inoculation amount had the most significant impact on the denitrification performance of strain SK-4. Moreover, Cu2+ at a mass concentration of 30－50 mg/L did not significantly affect the denitrification efficiency of strain SK-4, demonstrating its strong tolerance to Cu2+. The response surface test revealed that under conditions of pH 7.83, an inoculation amount （volume fraction） of 3.95%, and an oscillation speed of 149.21 r/min, strain SK-4 can achieve an optimal removal rate of NH+4-N of 82.33%. In the actual wastewater pilot test, even under the stress of 35.31 mg/L Cu2+, strain SK-4 could still efficiently degrade pollutants, with removal rates of TN, NH+4-N, NO－3-N, and COD reaching 93.79%, 98.52%, 85.42%, and 89.90%, respectively. This study optimized the nitrogen metabolism efficiency of strain SK-4 in copper-containing wastewater, verified its practical application potential, and provided a new treatment approach for the biological treatment of copper-containing wastewater.

Key words: biological denitrification, heterotrophic nitrification-aerobic denitrification, response surface, copper-containing wastewater, industrial water treatment

中图分类号: