生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (1): 67-.doi: 10.3969/j.issn.2095-1736.2025.01.067

• 研究报告 • 上一篇    下一篇

大麦HvGPAT5基因的克隆与表达分析

马健芝1， 杨成兰1， 杜明阳1， 罗天蓉1， 多杰措2， 熊辉岩2， 段瑞君1   

  1. 1. 青海大学 生态环境工程学院， 西宁 810000； 2. 青海大学 农牧学院， 西宁  810000
  • 出版日期:2025-02-18 发布日期:2025-02-12
  • 通讯作者: 段瑞君，博士，教授，研究方向为大麦驯化与适应机制，E-mail:ruijunduan@163.com
  • 作者简介:马健芝，硕士研究生，研究方向为植物生态学，E-mail:1499071396@qq.com
  • 基金资助:
    青海省科技厅科技特派员专项（2023-NK-P73）； 青海省“昆仑英才·高端创新创业人才”项目

Cloning and expression analysis of HvGPAT5 gene in barley

MA Jianzhi1, YANG Chenglan1, DU Mingyang1, LUO Tianrong1, DUO Jiecuo2,XIONG Huiyan2, DUAN Ruijun1   

  1. 1. College of Eco-Environmental Engineering, Qinghai University, Xining 810000, China;
    2. College of Agriculture and Animal Husbandry, Qinghai University, Xining 810000, China
  • Online:2025-02-18 Published:2025-02-12

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摘要： 甘油-3-磷酸酰基转移酶（Glycerol-3-phosphateacyltransferase，GPAT）是一类脂质生物合成过程中的关键酶，在植物生长发育和抗逆性相关的基本细胞代谢中起重要作用。以大麦‘Morex’为材料，克隆得到HvGPAT5基因，并对其进行生物信息学以及非生物胁迫下表达水平的分析。结果表明：HvGPAT5编码区全长1203 bp，编码400个氨基酸，编码蛋白为碱性不稳定疏水蛋白，具有4个跨膜结构域，亚细胞定位预测位于细胞膜；保守结构域分析推测，HvGPAT5蛋白具有酰基转移酶结构域，是典型的GPAT家族基因；系统进化结果表明，HvGPAT5与小麦同源蛋白的亲缘关系最近，与拟南芥ATS1归于同一亚家族；蛋白互作分析显示，HvGPAT5作为核心基因与大麦GPAT蛋白家族其他成员存在密切的相互作用关系。时空表达分析表明，该基因在叶中表达量相对较高，但在其他各组织中均呈现较低表达量。同时该基因在逆境胁迫下（干旱、盐、低温）根和叶中相对表达量均显著上调，说明HvGPAT5基因积极参与大麦的非生物胁迫响应并可能发挥正调控作用。研究为后续深入探索HvGPAT5基因的生物学功能研究奠定基础，也为大麦的抗逆性研究提供候选基因。

关键词: 大麦, HvGPAT5, 基因克隆, 非生物胁迫, 表达分析

Abstract: Glycerol-3-phosphateacyltransferase (GPAT) is a key enzyme in lipid biosynthesis that plays an important role in basic cellular metabolism related to plant growth and development and stress tolerance. In this study, theHvGPAT5gene in the barley (Hordeum vulgareL.) ‘Morex’ was cloned and subjected to bioinformatics analysis and relative expression analysis under abiotic stress. The results showed that the coding region ofHvGPAT5was 1203 bp in length, encoding a peptide of 400 residues. The protein was proposed to be a basic unstable hydrophobic protein and consisted of four transmembrane domains. Subcellular localization analysis indicated thatHvGPAT5was distributed in cell membrane. The conserved domain analysis showed HvGPAT5 contained a conserved acyltransferase structural domain, which was a typical member of the GPAT family. The phylogenetic analysis presented that HvGPAT5 had the closest relationship with homologues in the wheat, which belonged to the same subfamily asArabidopsis ATS1. Protein interaction analysis indicatedHvGPAT5acted as a core gene that interacted closely with other members of the barley GPAT protein family. Spatiotemporal expression analysis showed thatHvGPAT5had relatively high expression in leaves, but low expression in other tissues. Meanwhile, the relative expression ofHvGPAT5was significantly up-regulated in both roots and leaves under abiotic stress (drought, salt, and low temperature), indicating thatHvGPAT5possibly played a positive regulatory role in the abiotic stress response of barley. This study laid a foundation for the further exploration of the biological functions ofHvGPAT5and provided a candidate gene for the study of barley abiotic stress tolerance.

Key words: Hordeum vulgare;HvGPAT5, gene cloning, abiotic stress, expression analysis

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