生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (1): 76-.doi: 10.3969/j.issn.2095-1736.2025.01.076

• 研究报告 • 上一篇    下一篇

基于COI和ND5基因序列的新疆黑腿星翅蝗种群遗传多样性与分化

张　悦， 李金星， 王　阳， 任金龙， 赵　莉   

  1. 新疆农业大学 农学院 农林有害生物监测与安全防控重点实验室， 乌鲁木齐 830052
  • 出版日期:2025-02-18 发布日期:2025-02-12
  • 通讯作者: 赵莉，教授，研究方向为害虫综合治理，E-mail:782122497@qq.com；任金龙，副教授，研究方向为昆虫分类和系统进化、昆虫与植物互作、草原昆虫多样性及分布格局机制，E-mail:rjlinsect@163.com；赵莉和任金龙为共同通信作者
  • 作者简介:张悦，硕士，研究方向为农业昆虫与害虫防治，E-mail:1550752421@qq.com
  • 基金资助:
    科技基础资源调查专项（2019FY100403）

Genetic diversity and differentiation of Calliptamus barbarus barbarus(Costa)(Orthoptera: Acrididae) in Xinjiang on the basis of COI and ND5 sequences

ZHANG Yue, LI Jinxing, WANG Yang, REN Jinlong, ZHAO Li   

  1. Key Laboratory for Monitoring and Safety Prevention and Control of Pests in Agriculture and Forestry,
    College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China
  • Online:2025-02-18 Published:2025-02-12

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摘要： 为探究新疆黑腿星翅蝗(Calliptamus barbarus barbarus)不同地理种群的亲缘关系和遗传距离，选取12个地理种群109条线粒体COI和ND5基因序列作为分子标记进行测序。通过联合两个基因分析该蝗的种群遗传多样性和遗传分化情况，探讨遗传多样性与气象因素和环境因素的相关性。结果显示：黑腿星翅蝗12个地理种群COI和ND5联合基因表现出高遗传多样性（Hd为0.9333~1.0000，Pi为0.0606~0.3253），各种群间多存在中度遗传分化，基因交流较为密切（Fst为0.1062，Nm为2.11）。AMOVA分析显示种群间的遗传差异来源于种群内部（89.68%），Mantel检验显示遗传分化Fst与地理距离存在显著正相关关系（R=0.4034，P=0.037）。黑腿星翅蝗遗传多样性的主要影响因素是海拔，这将为后续解析该蝗在新疆的种群进化历史提供理论依据。

关键词: 黑腿星翅蝗, 地理种群, COI, ND5, 遗传多样性, 遗传分化

Abstract: To investigate the affinities and genetic mechanisms of distances geographical populations ofCalliptamus barbarus barbarus, 109 mitochondrial COI and ND5 gene sequences from 12 geographic populations ofC.barbarus barbaruswere sequenced. The genetic diversity and the differentiation of this grasshopper in Xinjiang were analyzed by these two genes. By examining the combination of genes, the link between genetic diversity and meteorological and environmental factors was explored. The results revealed a substantial genetic diversity (Hd:0.9333－1.0000,Pi:0.0606－0.3253) in the COI and ND5 genes across the 12 geographic populations, with moderate genetic differentiation among these populations and relatively strong gene flow (Fst:0.1062,Nm:2.11). The AMOVA analyses found that the majority (89.68%) of genetic variation was observed within populations, and the Mantel test established a significant positive correlation betweenFstand geographic distance (R=0.4034,P=0.037). Altitude emerged as the primary factor influencing the genetic diversity ofC. barbarus barbaruspopulations, thus providing a theoretical foundation for subsequent analysis of the grasshopper evolutionary history in Xinjiang.

Key words: Calliptamus barbarus barbarus, geographic population, COI, ND5, genetic diversity, genetic differentiation

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