生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (6): 88-.doi: 10.3969/j.issn.2095-1736.2023.06.088

• 研究报告 • 上一篇    下一篇

炭药提取液对小鼠皮肤伤口愈合过程中胶原组成的影响

王俊杰1,2， 高建萍2， 张　扬2， 张贵锋2， 马　莉1,3   

  1. 1. 成都中医药大学 药学院， 成都 611137； 2. 中国科学院过程工程研究所生化工程国家重点实验室，
    北京 100190； 3. 首都医科大学 中医药学院， 北京 100069
  • 出版日期:2023-12-18 发布日期:2023-12-18
  • 通讯作者: 张贵锋，博士，研究员，研究方向为蛋白质分析检验，E-mail:gfzhang@ipe.ac.cn；马莉，博士，教授，研究方向为动物类炮制原理及质量评价，E-mail:marytcm@ccmu.edu.cn;张贵锋和马莉为共同通信作者
  • 作者简介:王俊杰，硕士研究生，研究方向为中药新制剂、新剂型、新技术应用，E-mail:2020zs425@stu.cdutcm.edu.cn
  • 基金资助:
    国家重点研发计划项目（No.2021YFC2400804）

Effects of charcoal medicine extract on collagen composition in skin wound healing of mice #br#

WANG Junjie1,2, GAO Jianping2, ZHANG Yang2, ZHANG Guifeng2, MA Li1,3   

  1. 1. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;
    2. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Beijing 100190, China;
    3. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
  • Online:2023-12-18 Published:2023-12-18

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摘要： 研究炭药提取液(液体敷料1与液体敷料2)对小鼠皮肤伤口愈合过程中I、III胶原蛋白含量变化的影响。以小鼠全皮层创伤为模型，将液体敷料1、液体敷料2涂抹于小鼠背部伤口，给药3、6、9、13、17和21 d后，通过液质联用法测定小鼠创面组织中I、III型胶原含量。结果表明：液体敷料1与液体敷料2能明显提高创面愈合率并加速结痂掉落，并在第9、13天明显增加总胶原的含量（P<0.01）；第3、9天，液体敷料1与液体敷料2组的I、III型胶原含量均大于空白组（P<0.05）；且在第17、21天，两组胶原I/III的比例均大于空白组（P<0.01）。研究为探索炭药提取液促进伤口愈合的机理提供基础。

关键词: 炭药提取液, 创面愈合, I型胶原, III型胶原, 成纤维细胞

Abstract: The study determined the effect of charcoal medicine extract (liquid dressing 1 and liquid dressing 2) on the changes of type I and III collagen content in the healing process of skin wounds in mice. A model of full-thickness wound in mice was established. Liquid dressing 1 and liquid dressing 2 were applied daily on the back of mice. After 3, 6, 9, 13, 17 and 21 days of administration, the content of collagen I and III in wound tissues of mice was determined by HPLC-MS. Results showed that liquid dressing 1 and liquid dressing 2 can obviously improve the wound healing rate and accelerate the scab shedding. Compared with the control group, liquid dressing 1 and liquid dressing 2 could obviously promote the synthesis of total collagen at 9 d and 13 d(P<0.01). The content of type I and type III collagen in liquid dressing 1 and liquid dressing 2 were greater than that of control group at 3 d and 9 d (P< 0.05). The ratio of collagen I/III in liquid dressing 1 and liquid dressing 2 were greater than that of control group at 17 d and 21 d (P<0.01). This study provided a theoretical basis for the treatment of wound healing with charcoal medicine extract.

Key words: charcoal medicine extract, wound healing, type I collagen, type III collagen, fibroblast

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