生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (2): 61-.doi: 10.3969/j.issn.2095-1736.2021.02.061

• 研究报告 • 上一篇    下一篇

暗褐网柄牛肝菌的分子鉴定及母种培养基筛选

  

  1. 1.四川大学生命科学学院生物资源与生态环境教育部重点实验室，成都610065；2.四川省自然资源科学研究院，成都610015
  • 出版日期:2021-04-18 发布日期:2021-04-25
  • 通讯作者: 秦小波，博士，副研究员，主要从事植物与微生物资源开发研究，Email:qxb_2003@163.com;徐莺，博士，副教授，主要从事植物遗传和微生物研究，Email:xuying@scu.edu.cn
  • 作者简介:张冰冰，硕士研究生，主要从事土壤生态系统保护与微生物资源利用研究，Email:2425580236@qq.com
  • 基金资助:
    国家自然科学基金（31400288）；四川省青年科学技术基金（2015JQ0052）；四川省科技计划项目

Molecular identification and selection of mother culture media of Phlebopus portentosus

  1. 1. Key Laboratory of Bio⁃Resources and Eco⁃Environment，Ministry of Education，College of Life Sciences，Sichuan University，Chengdu 610065，China；2. Sichuan Natural Resources Research Institute，Chengdu 610015，China
  • Online:2021-04-18 Published:2021-04-25

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摘要： 为保护暗褐网柄牛肝菌野生资源及促进其人工栽培技术，对采自中国西南地区的子实体样品进行形态学初步鉴定和分子鉴定。随后，对其母种培养基的氮源及其浓度、无机盐类型进行筛选并探究维生素B1和不同氮源结合时对菌丝生长的影响。结果表明：供试菌株均为暗褐网柄牛肝菌（Phlebopusportentosus），最佳氮源为NH4Cl，最适氮源质量浓度为0.5 g/L；维生素B1对暗褐网柄牛肝菌菌丝生长的影响差异较大，仅与硝酸铵组合时显著促进菌丝生长，与其余3种氮源组合时无显著促进甚至抑制菌丝生长的效果。实验的两种无机盐组合对菌丝生长无显著差异，但从经济成本的角度出发，建议采用MgCl2／KCl组合。优化后的培养基组成为：葡萄糖，20 g；NH4Cl，0.5 g；MgCl2，1 g；KCl，1 g；马铃薯，200 g；琼脂，20 g；H2O，1 L。

关键词: 暗褐网柄牛肝菌, 分子鉴定, 菌落直径, 培养基, 筛选

Abstract: In order to protect the wild resources ofPhlebopusand promote its artificial cultivation process，the morphological and molecular identification of the fruit body samples collected from Southwest China were carried out. Then，the nitrogen source and its concentration，inorganic salt type added into parent medium were screened，and the effect of the combination of vitamin B1 and different nitrogen sources on the mycelial growth was explored. The results showed that the tested strains were allPhlebopusportentosus.The best nitrogen source was ammonium chloride，and the best nitrogen source concentration was 0.5 g/L. The effect of vitamin B1 on the growth of mycelium was significant，only when combined with ammonium nitrate，it significantly promoted the growth of hypha，but not when combined with the other three nitrogen sources. There was no significant difference in mycelium growth between the two inorganic salt combinations，but from the perspective of economic cost，MgCl2/KCl combination was recommended. The optimal compositions were as follows：glucose，20 g；NH4Cl，0.5 g；MgCl2，1 g；KCl，1 g；potato，200 g；agar，20 g；H2O，1 L.

Key words: Phlebopusportentosus；molecular identification；colony diameter；medium；screening  ,

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