生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (2): 65-.doi: 10.3969/j.issn.2095-1736.2021.02.065

• 研究报告 • 上一篇    下一篇

一株产吲哚乙酸菌的筛选、鉴定及培养条件优化

  

  1. 1.中国科学院城市环境研究所，厦门361021；2.中国科学院大学，北京100049
  • 出版日期:2021-04-18 发布日期:2021-04-25
  • 通讯作者: 朱葛夫，博士，研究员，研究方向为厌氧功能微生物及其利用，Email:gfzhu@iue.ac.cn
  • 作者简介:周铭典，硕士研究生，专业方向为农业生物质废弃物利用，Email:mdzhou@iue.ac.cn
  • 基金资助:
    国家自然科学基金项目（51678553、21876167、21477122）；国家重点研发计划项目（2018YFD0500202-04）;福建省自然科学基金项目（2019J05161）

Screening，identification and culture condition optimization of an indole-3-acetic acid producing bacterial strain

  1. 1. Institute of Urban Environment，Chinese Academy of Sciences，Xiamen 361021，China；2. University of Chinese Academy of Sciences，Beijing 100049，China
  • Online:2021-04-18 Published:2021-04-25

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摘要： 通过平板涂布培养法和Salkowski比色法对堆肥中具有产吲哚乙酸（IAA）能力的菌株进行初步筛选，并进一步利用分光光度法定量复筛其中具有高产IAA能力的菌株。试验共分离得到3株具有产IAA能力的菌株，其中菌株29B具有较强分泌IAA的能力，培养96 h时IAA产量达到54.73μg/mL。通过菌株形态观察、部分生理生化特性测定及16S rDNA基因序列分析对菌株29B进行分类鉴定，初步确定菌株29B为停滞棒杆菌（Corynebacteriumstationis）。设计单因素试验以IAA含量为评价指标，对菌株29B产IAA能力进行条件优化。结果表明，菌株29B最佳培养条件是初始pH值为7.0，接种量（体积分数）为2%，35 ℃摇床培养108 h。在此最优条件下，菌株29B的IAA产量可达138.87μg/mL，比优化前提高153.74 %。研究结果为菌株29B在农业生产中的应用提供了实验基础和理论依据。

关键词: 吲哚乙酸（IAA）, 筛选, 条件优化, 堆肥

Abstract: The indole-3-acetic acid（IAA）-producing strains were preliminarily screened by using spread plate and Salkowski colorimetric method，and quantitative analysis of spectrophotometry was used to rescreen the strain with high IAA producing capacity. Three IAA-producing strains were isolated from the compost sample，and strain 29B showed the highest IAA productivity with the yield reached 54.73 μg/mL in 96 h of cultivation. Based on morphological observation，part of physio-biochemical characteristics and analysis of 16S rDNA gene sequences，the strain 29B was identified asCorynebacteriumstationis. The single factor tests were designed to optimize the IAA production of strain 29B. Results showed that the optimum culture conditions were as follows：initial pH value，7.0；inoculum amount（volume fraction），2%；shaking culture at 35 ℃ for 108 h. Under the optimal culture conditions，the yield of IAA reached up to 138.87 μg/mL，which was increased by 153.74% compared with that before optimization. These results would provide an experimental and theoretical basis for the application of strain 29B in agricultural production.

Key words: indole-3-acetic acid（IAA）, screening, optimization, compost

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