生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (2): 70-.doi: 10.3969/j.issn.2095-1736.2021.02.070

• 研究报告 • 上一篇    下一篇

三峡库区香溪河沉积物厌氧氨氧化菌的时空分布

  

  1. 1.三峡大学湖北省生物酵素工程技术研究中心，宜昌443000； 2.三峡大学水利与环境学院，宜昌443000；3.三峡大学生物与制药学院，宜昌443000
  • 出版日期:2021-04-18 发布日期:2021-04-25
  • 通讯作者: 李　宁，硕士，副教授，主要从事环境微生物研究， E⁃mail：583392532@qq.com
  • 作者简介:赵折红，硕士研究生，主要从事环境微生物研究， E⁃mail：1547941734@qq.com
  • 基金资助:
    国家自然基金青年基金项目（31500422）；湖北省技术创新专项（重大项目）（2019ABA114）

Temporal and spatial distribution of anaerobic ammonium oxidizing bacteria in sediments of Xiangxi River of the Three Gorges Reservoir Area

  1. 1. Hubei Province Biological Enzyme Engineering Technology Research Center，China Three Gorges University，Yichang 443000，China；2. College of Water Resources and Environment，China Three Gorges University，Yichang 443000，China；3. College of Biological and Pharmaceutical Sciences，China Three Gorges University，Yichang 443000，China
  • Online:2021-04-18 Published:2021-04-25

PDF (PC)

99

摘要： 为了解三峡库区香溪河段沉积物中厌氧氨氧化菌（AnAOB）的时空分布，利用实时荧光定量PCR技术（qPCR）检测香溪河5位点沉积物中AnAOB丰度，并通过Spearman相关性分析探讨理化因子对AnAOB丰度的影响。结果表明，香溪河沉积物中存在AnAOB，丰度介于1.45×104~3.16×109 g-1，不同位点不同时间AnAOB的数量有较大变化。时间上（2016年沉积物0~10 cm），5位点的AnAOB丰度皆在秋季最高，a和e位点显示同样的分布规律，即秋季>冬季>春季>夏季。空间上（0~2 cm），2016年春冬两季AnAOB丰度在e位点最高，夏秋两季在d位点最高。2017年香溪河沉积物AnAOB丰度为b>a>e>c>d，夏季e>c>d>a>b，冬季c>d>e>b>a；d和e位点（0~10 cm）有随着沉积物深度增加，AnAOB丰度先增加后降低的趋势。Spearman相关分析证实，AnAOB丰度与沉积物中总氮（TN）（P<0.01）、硝态氮和有机碳（TOC）（P<0.05）浓度显著相关；双因素方差分析结果证明不同理化因子和不同季节下AnAOB丰度无显著性差异（P<0.05）。

关键词: AnAOB, 实时荧光qPCR, 时空分布

Abstract: In order to understand the spatial and temporal distribution of anammox bacteria（AnAOB）in the sediments of Xiangxi River in the Three Gorges Reservoir Area，the abundance of AnAOBwasdetected by real⁃time quantitative PCR（qPCR）in the sediments of the five sites of Xiangxi River，and the influence of physicochemical factors on AnAOB abundance was analysed by Spearman rank correlations. The results showed that AnAOB existed in the sediments of Xiangxi River，and its abundance was between 1.45×104-3.16×109 g-1sediment. The number of AnAOBs varied greatly at different sites. In terms of time（0-10 cm in 2016），the AnAOB abundance of the five loci was highest in autumn，and the sites（a and e）showed the same distribution pattern，namely autumn>winter>spring>summer. In terms of space（0-2 cm in 2016），AnAOB abundance was highest at the site（e）in the spring and winter，and highest in the site（d）in the summer and autumn. In spring of 2017，AnAOB abundance was b>a>e>c>d，e>c>d>a>b in summer，c>d>e>b>a in winter；As the sediment depth increased，the AnAOB abundance increased firstly and then decreased in the d and e sites（0-10 cm）. Spearman rank correlations confirmed that AnAOB abundance was significantly correlated with total nitrogen（TN）（P<0.01），nitrate nitrogen and total organic carbon（TOC）（P<0.05）concentrations of insediments. AnAOB abundance under different physicochemical factor and seasons showed no significant difference by two-way analysis of variance（P<0.05）.

Key words: AnAOB, real?time quantitative PCR, temporal and spatial distribution

中图分类号: