生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (2): 75-.doi: 10.3969/j.issn.2095-1736.2021.02.075

• 研究报告 • 上一篇    下一篇

濒危植物大花黄牡丹种胚快速成苗技术研究

  

  1. 1.西藏农牧学院高原生态研究所，林芝860000； 2.西藏农牧学院⁃西南大学药用植物联合研发中心，林芝860000； 3.西藏农牧学院食品科学学院，林芝860000
  • 出版日期:2021-04-18 发布日期:2021-04-25
  • 通讯作者: 禄亚洲，博士，讲师，研究方向为药用植物分子生物学，Email:luyazhou001@126.com
  • 作者简介:尹秀，硕士，讲师，研究方向为药用植物分子生物学，Email:yinxiu111@163.com
  • 基金资助:
    中央本级重大增减支项目（2060302）；西藏自治区科技计划项目科技重大专项（XZ201901-GA-04）；西藏自治区林芝市2018年地校合作项目。

Study on rapid seedling⁃raising technology of embryos of the endangered species Paeonia Ludlowii

  1. 1.Research Institute of Plateau Ecology，Tibet Agriculture and Animal Husbandry University，Nyingchi 860000，China； 2.Tibet Agriculture and Animal Husbandry College⁃Southwest University，Medicinal Plants Joint Research and Development Centre，Nyingchi 860000，China；3.Food Science College，Tibet Agriculture and Animal Husbandry University，Nyingchi 860000，China
  • Online:2021-04-18 Published:2021-04-25

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摘要： 探索将植物组织培养技术运用于大花黄牡丹的繁育，以期提高其成苗率并缩短育苗周期。将分离出的种胚直接接种到添加有植物生长调节剂的MS培养基上，研究其萌发率和成苗率变化情况。结果表明种胚培养的最优激素配比为：MS+0.5 mg/L 6⁃BA+0.5 mg/L IAA+0.2 mg/L GA3，萌发率和成苗率达到最大值，分别为72.25%和70%。将种胚接种到上述培养基上培养，3~5 d种胚开始萌动，7 d种胚萌发，15 d子叶抽出，长1~4 cm，30 d胚根抽出，然后将大花黄牡丹的无菌苗转接到生根培养基上（1/2 MS+1 mg/L IBA+0.2 mg/L NAA）培养生根，待无菌苗苗龄达到2~3个月，幼苗高约5 cm时可先炼苗一周，然后移栽，2~3个月成苗，与周期为两年的传统播种育苗相比，大大缩短了育种周期。

关键词: 大花黄牡丹, 植物组织培养, 种胚, 成苗率

Abstract: In this study，the plant tissue culture technology was applied to the breeding ofPaeonialudlowiiin order to improve the seedling rate and shorten the seedling cycle. The seed coat and endosperm were stripped，the seed embryo was separated and inoculated directly into MS medium with plant growth regulators，and then the changes of germination rate and seedling rate were studied. The results showed that the optimal hormone ratio of embryo culture was as follows：MS+0.5 mg/L 6⁃BA+0.5 mg/L IAA+0.2 mg/L GA3，the germination rate and seedling rate reached the maximum，72.25% and 70%，respectively. When the embryos were inoculated on the above medium，the seed embryos began to germinate after 3 to 5 days，the seed embryos germinated after 7 days，the cotyledons were emerged from the cotyledons after 15 days，radicle was formed after in 30 days，about 1⁃4 cm in length and the aseptic seedlings ofP.ludlowiiwere transferred to the rooting medium for 1/2 MS+1 mg/L IBA+0.2 mg/L NAA to take root. When the aseptic seedlings were 2 to 3 months old and about 5 cm in height，the seedlings could be scoured for one week and then transplanted and the seedling formation period was 2 to 3 months，which greatly reduced the breeding cycle compared with the traditional seeding cycle of two years.

Key words: Paeonialudlowii, plant tissue culture, seed embryo, seedling rate

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