生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (2): 46-.doi: 10.3969/j.issn.2095-1736.2021.02.046

• 研究报告 • 上一篇    下一篇

十二烷基羟丙基磺基甜菜碱对表皮葡萄球菌生物被膜的影响

  

  1. 1.上海交通大学医学院附属国际和平妇幼保健院，上海200030；2.安徽中医药大学中西医结合学院，合肥230012；3.江苏海洋大学药学院，连云港222005
  • 出版日期:2021-04-18 发布日期:2021-04-25
  • 通讯作者: 官　妍，教授，硕士生导师，研究方向为抗细菌生物被膜， E⁃mail：guanyan0726@sina.com
  • 作者简介:石晶金，公共卫生医师，硕士，研究方向为医院获得性感染， E⁃mail：shijingjin1115@sina.cn
  • 基金资助:
    安徽省教育厅自然科学研究重点项目（KJ2019A0433）；上海交通大学医学院附属国际和平妇幼保健院院级课题（YN201806）

The effect of dodecyl hydroxypropyl sulfobetaine on biofilm of Staphylococcus epidermidis

  1. 1. International Peace Maternal and Child Health Hospital，School of Medicine，Shanghai Jiao Tong University，Shanghai 200030，China；2. College of Integrated Traditional Chinese and Western Medicine，Anhui University of Chinese Medicine，Hefei 230012，China；3. School of Pharmacy，Jiangsu Ocean University，Lianyungang 222005，China
  • Online:2021-04-18 Published:2021-04-25

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摘要： 通过表面活性剂十二烷基羟丙基磺基甜菜碱（Dodecyl hydroxypropyl sulfobetaine，DSB）对表皮葡萄球菌生物被膜内细菌代谢、生物被膜的清除效率和多糖胞间黏附素（Polysaccharide intercellular adhesion，PIA）形成等作用指标的检测，为DSB防止表皮葡萄球菌生物被膜污染提供理论及实践依据。以万古霉素为阳性对照，利用XTT减低法，评价DSB对表皮葡萄球菌生物被膜内细菌代谢的影响及对已形成的生物被膜的清除效率。采用刚果红培养基，观察DSB对PIA产生的影响，用激光共聚焦显微镜观察DSB对生物被膜作用的效果。结果显示，质量浓度为1 024、512、256和128 mg/L的DSB对ATCC 35984生物被膜内细菌的代谢有极显著影响（P<0.001）；质量浓度依次为64、32、16和8 mg/L的DSB在6、12和24 h对表皮葡萄球菌生物被膜均有显著的清除作用（P<0.001）；质量浓度为16和8 mg/L的DSB对PIA形成无明显影响。结果表明，DSB在影响表皮葡萄球菌生物被膜内细菌代谢及清除已形成的生物被膜等环节均可发挥显著作用。

关键词: 十二烷基羟丙基磺基甜菜碱, 表皮葡萄球菌, 生物被膜, 代谢, PIA, 清除效率

Abstract: To analyse the effect of dodecyl hydroxypropyl sulfobetaine（DSB）on bacterial metabolism，clearance efficiency and formation of polysaccharide intercellular adhesion ofStaphylococcusepidermidisbiofilm and to provide reliable theory and practical evidence for DSB to prevent biofilm contamination ofS.epidermidis，vancomycin was used as positive control，XTT reduction was used to evaluate the clearance and metabolism of DSB onS.epidermidisbiofilm，Congo red medium and laser scanning confocal microscope（LSCM）were used to observe the effect of DSB on PIA and biofilm，respectively. The results showed that 1 024，512，256 and 128 mg/L of DBS had significantly effect on metabolism of ATCC 35984，and 64，32，16 and 8 mg/L of DBS had significantly effect on clearance in the 6，12and24 hours post treatment（P<0.001）. However，16 and 8 mg/L of DSB had no significant effect on PIA formation. These results indicated that DSB significantly affects bacterial metabolism in biofilms ofS.epidermidisand in scavenging formed biofilm.

Key words: dodecyl hydroxypropyl sulfobetaine, Staphylococcusepidermidis, biofilm, metabolism, polysaccharide intercellular adhesion（PIA）, clearance

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