生物学杂志

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几种微藻饵料对缢蛏稚贝脂质营养组成的影响

  

  1. 1. 宁波大学海洋学院 应用海洋生物技术教育部重点实验室, 宁波 315211;
    2. 福建省宝智水产科技有限公司, 漳州 363503
  • 出版日期:2016-06-18 发布日期:2016-06-18
  • 通讯作者: 徐继林,研究员,博士生导师,研究方向为海洋生物的环境生态和养殖生态学研究,E-mail: xujilin@nbu.edu.cn
  • 作者简介:周率,硕士研究生,从事海洋生物化学研究,E-mail: zhoulv9597@126.com
  • 基金资助:
    国家自然科学基金(31172448);教育部博士点基金优先发展领域项目(20133305130001);浙江省公益性项目(2014C32081);浙江自然科学基金项目(LY15C190004);宁波市科技攻关项目(2013C10014,2014C10005)

Effect of several diets on the composition of fatty acids and sterols in juvenile Sinonovacula constricta

  1. 1. Key Laboratory of Applied Marine Biotechnology, School of Marine Science, Ningbo University, Ningbo
    315211;
     2. Zhibao Fishery Science Co., Ltd. in Fujian Province, Zhangzhou 363503, China
  • Online:2016-06-18 Published:2016-06-18

摘要:

通过用4种缢蛏人工养殖中常用的饵料微藻(角毛藻、球等鞭金藻、威氏海链藻、假微型海链藻),以单种饵料和混合饵料的形式对缢蛏稚贝进行投喂实验,研究不同的微藻饵料对缢蛏稚贝的脂肪酸和甾醇组成的影响。对喂食饵料后的缢蛏稚贝进行脂肪酸甾醇的GC-MS分析,共检测到16种脂肪酸和7种甾醇,不同的单种饵料及混合饵料的缢蛏投喂组的脂肪酸、甾醇差异很大。C16:2在含有威氏海链藻的单种饵料和混合饵料的投喂组被检测到;金藻混合藻饵料的缢蛏投喂组脂肪酸种类最丰富(13~15种),PUFA百分含量最高,胆甾醇含量不高但富含菜子甾醇。因此,从微藻饵料对缢蛏稚贝的脂质营养化影响上来说,金藻的混合饵料是最佳饵料。

关键词:  , 微藻;缢蛏;脂肪酸;甾醇;气象色谱-质谱连用分析

Abstract:

It is investigated that effects of the four microalgae (Chaetoceros calcitrans, Isochrysis galbana, Thalassiosira pseudonana and Thalassiosira weissflogii) and their mixture, which commonly used as S. constricta rearing diets, on the composition of fatty acids and sterols in juvenile S. constricta. The fatty acid and sterol composition of juveniles were analyzed by GC-MS. The results showed that different diets have effect on fatty acid and sterol compositions in juveniles, and 16 fatty acids and 7 sterols have been detected. C16:2 detected in T. weissflogii and its mix. Juveniles fed with I. galbana mix was abundant in the species of fatty acids(13-15) and C28:1(Δ5/24Me) (Brassicasterol). Besides, the PUFA percentage content of Juveniles fed with I. galbana mix was higher than that of others. Above all, we choose I. galbana mix as optimal diet because they made lipids of S. constricta juveniles more nitrification.

Key words: microalgae, S. constricta, fatty acid, sterol, GC-MS