生物学杂志

生物学杂志

• 研究报告 • 上一篇    下一篇

Na2ATP对后肢制动大鼠肌梭胞内游离钙的影响

  

  1. 1. 西安医学院 基础医学部， 西安 710021； 2. 湖北文理学院 医学院， 襄阳 441053；3. 西安交通大学 医学部, 西安 710016
  • 出版日期:2019-08-18 发布日期:2019-08-18
  • 通讯作者: 李雪萍，博士，教授，研究方向为神经内分泌学，E-mail: lxp_lucy@qq.com
  • 作者简介:余蕾，硕士，讲师，研究方向为生理学，E-mail：yulei@xiyi.edu.cn
  • 基金资助:
    国家自然科学基金青年基金项目（项目编号31300981）; 陕西省自然科学基础研究计划资助项目（项目批准号 2012JM4014）

Effects of adenosine triphosphate on intracellular free calcium in muscle spindle of hind limb immobilization rats

  1. 1. Department of Basic Medical, Xi′an Medical University, Xi′an 710021; 2. Medical College, Hubei University of Arts and Science， Xiangyang 441053; 3. Department of Medical, Xi′an Jiaotong University, Xi′an 710016, China
  • Online:2019-08-18 Published:2019-08-18

PDF (PC)

122

摘要： 采用激光扫描共聚焦显微镜技术，观察大鼠后肢制动一周比目鱼肌梭内肌细胞内静息Ca2+的变化，并以Na2ATP作为对抗措施，观察其对后肢制动大鼠梭内肌细胞Ca2+的影响。结果显示后肢制动一周，大鼠比目鱼肌梭内肌细胞Ca2+浓度明显升高，而经Na2ATP注射后，大鼠后肢制动一周梭内肌细胞Ca2+浓度升高不明显。提示Na2ATP可对抗后肢制动所致的梭内肌细胞钙超载。为大鼠肌梭生理的研究及钙离子与废用性肌萎缩的实验研究提供了有意义的资料。

关键词: Na2ATP；Ca2+；肌梭；比目鱼肌 

Abstract: The confocal laser scanning microscopy was adopted to observe the changes of resting Ca2+ in soleus intrafusal muscle cells of rats after hindlimb immobilization for a week, and the effect of Na2ATP on Ca2+ cells of intrafusal hindlimb immobilization in rats was observed. The results showed that the resting Ca2+ in soleus intrafusal muscle cells of rats increased significantly after hindlimb immobilization for a week, and after Na2ATP injection, the resting Ca2+ in soleus intrafusal muscle cells of rats had no obvious increase. The experiment suggests that Na2ATP can resist calcium overload of intrafusal muscle cell induced by hindlimb immobilization. This study provides valuable information for the study of rat muscle spindle physiology and the experimental study of calcium ion and weightlessness.

Key words: Na2ATP, Ca2+, muscle spindle, soleus muscle

中图分类号: