生物学杂志 ›› 2020, Vol. 37 ›› Issue (6): 38-.doi: 10.3969/j.issn.2095-1736.2020.06.038

• 研究报告 • 上一篇    下一篇

山竹果皮提取物“芒果醚”抑制乳腺癌细胞MCF-7增殖与迁移

  

  1. 东华大学 化学化工与生物工程学院, 上海 201620
  • 出版日期:2020-12-18 发布日期:2020-12-21
  • 通讯作者: 张云龙,博士,副教授,研究方向为分子与肿瘤药理学,E-mail:zhyl114790@163.com;邵志宇,博士,副教授,研究方向为天然药物化学,E-mail:zyshao@dhu.edu.cn
  • 作者简介:黄凯悦,硕士研究生,研究方向为分子与肿瘤药理学,E-mail:18226212391@163.com
  • 基金资助:
    国家自然科学基金(No. 81671508;No. 31000352);上海市科委“一带一路”国际合作项目(19410741800);校企合作项目(No.HX105180781);上海市大学生创新创业计划项目(No.105-03-0178139;105-03-0178229)

Mangosteen peel extract "Mango Ether" inhibits proliferation and migration of breast cancer cell MCF-7#br#

  1. College of Chemistry Chemical Engineering and Bioengineering, Donghua University, Shanghai 201620, China
  • Online:2020-12-18 Published:2020-12-21

摘要: 从山竹(Garcinia mangostana)果皮内提取和鉴定了一种黄酮类化合物“芒果醚”,虽已证实其具有抑瘤作用,但机制不清。为了检测此芒果醚对乳腺癌细胞增殖抑制效果,以正常人乳腺细胞MCF-10作为对照,探析了其对MCF-7、SUM1315MO2和MDA-MB-231 3种乳腺癌细胞增殖的影响,证实其对MCF-7细胞具有剂量和时间依赖性抑制作用,24 h给药处理的IC50值为20 μmol/L,同时出现凋亡小体。蛋白质免疫印迹反应检测凋亡信号途径显示:Bcl-2剂量依赖性下调,而Bax上调,Cytochrome C显著上调,Precaspase-9也明显下调,这表明芒果醚通过促发线粒体依赖性凋亡途径而抑制了MCF-7增殖。另外,划痕实验检测结果显示,芒果醚可有效减弱MCF-7细胞迁移。山竹果皮提取物“芒果醚”具有抑制乳腺癌细胞MCF-7增殖与迁移作用,为其用于抗癌治疗开发提供了相关依据。

关键词: 芒果醚, 乳腺癌, 细胞增殖, 细胞凋亡, 细胞迁移

Abstract: A kind of flavonoid "Mango Ether" was extracted and identified from mangosteen peel, and its anti-tumor activities in breast cancer cells were proved. However, the mechanism of Mango Ether is unclear. Three kinds of breast cancer cells, MCF-7, SUM1315MO2 and MDA-MB-231 were used to detect anti-proliferation effects of mango ether, taken the normal human breast cell MCF-10 as control cells. As results, Mango Ether had anti-proliferation effect on MCF-7 cells in a dose-and time-dependent manner. Its IC50 value of 24 h was 20 μmol/L, accompanied with apoptotic bodies. The detection of apoptotic signaling pathway by Western Blot showed that the expression of Bcl-2 down-regulated in a dose-dependent manner, while Bax and Cytochrome C significantly up-regulated, as well as the precaspase-9 expression also down-regulated. These results indicated that Mango Ether inhibited MCF-7 cells proliferation by a mitochondria-dependent apoptotic pathway. In addition, the results of the scratch test showed that Mango Ether can effectively attenuate the migration of MCF-7 cells. In conclusion, mangosteen peel extract, Mango Ether, could inhibit the proliferation and migration of breast cancer MCF-7 cells, which provids useful evidence for anti-tumor treatment strategy.

Key words: Mango Ether, breast cancer, cell proliferation, apoptosis, cell migration

中图分类号: