生物学杂志

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低温大气压等离子体激活培养基选择性杀伤三阴性乳腺癌细胞

  

  1. 1. 江南大学 粮食发酵工艺与技术国家工程实验室, 无锡 214122;2. 江南大学 生物工程学院 工业生物技术教育部重点实验室, 无锡 214122;3. 江南大学 物联网工程学院, 无锡 214122; 4. 江南大学附属医院 肿瘤科, 无锡 214062
  • 出版日期:2019-06-18 发布日期:2019-06-18
  • 通讯作者: 戴晓峰,教授,研究方向为乳腺癌分型及肿瘤靶向治疗,E-mail:xiaofeng.dai@jiangnan.edu.cn
  • 作者简介:项良剑,硕士研究生,研究方向为肿瘤靶向治疗,E-mail: 18262280048@126.com
  • 基金资助:
    国家自然科学基金面上项目(基金编号31471251);江苏省自然科学基金面上项目(基金编号BK20161130);江苏省六大人才高峰项目(基金编号SWYY-128);江南大学医学院ESI培育专项(基金编号1286010241170320)

Cold atmospheric plasma-activated medium selectively inhibits triple negative breast cancer cells

  1. 1. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122; 2. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122; 3. Engineering Research Center of IoT Technology Applications, Jiangnan University, Wuxi 214122;4. Department of Oncology, Affiliated Hospital of Jiangnan University, Wuxi 214062, China
  • Online:2019-06-18 Published:2019-06-18

摘要: 低温大气压等离子体(CAP)近年来作为一种新兴的抗癌手段逐渐为人们所熟知。三阴性乳腺癌在多种乳腺癌亚型中恶性程度最高,由于缺少有效的治疗手段导致预后很差。发现等离子体激活的培养基(PAM)能够选择性抑制三阴性乳腺癌细胞增殖和迁移能力,而对正常细胞和非三阴性乳腺癌细胞影响较小,同时PAM能够抑制MDAMB231中原本高度激活的JNK的磷酸化程度。因此推测累积的基因组突变和高度激活的MAPK/JNK信号通路使三阴性细胞更容易被PAM杀死。研究提出了CAP杀伤作用与乳腺癌亚型之间的相关性,并且为探索CAP选择性杀死三阴性细胞的机制提供了前期基础。

关键词: 低温大气压等离子体, 乳腺癌, 细胞凋亡, 细胞迁移, JNK

Abstract: Cold atmospheric plasma(CAP) has become a promising onco-therapeutic approach in recent years. Triple negative breast cancer is most aggressive among various breast cancer subtypes and has worst prognosis due to lack of effective therapy. We found that plasma-activated medium(PAM) selectively inhibited the proliferation and migration of triple negative breast cancer cells while causing little effect on other subtypes, and PAM selectively inhibited the phosphorylation of JNK in MDAMB231. We suggested that it was the more genome mutation and hyper-activated MAPK/JNK pathway in triple negative breast cancer cells that made them more sensitive to CAP. Our work revealed the relation between CAP and breast cancer subtypes and provides a preliminary basis for exploring the mechanism of CAP selectively killing triple negative breast cancer cells. 

Key words: cold atmospheric plasma, breast cancer, cell apoptosis, cell migration, JNK

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