生物学杂志

生物学杂志 ›› 2022, Vol. 39 ›› Issue (1): 84-.doi: 10. 3969/ j. issn. 2095-1736. 2022. 01. 084

• 研究报告 • 上一篇    下一篇

光照强度对中缅树鼩行为、学习记忆和氧化应激的影响

  

  1. 1. 云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室, 云南师范大学生命科学学院, 昆明650500; 2. 云南经济管理学院, 昆明650106
  • 出版日期:2022-02-18 发布日期:2022-02-15
  • 通讯作者: 朱万龙,教授,研究方向为动物生理生态,E-mail:zwl_8307@163.com
  • 作者简介:彭鸿碧,硕士研究生,研究方向为动物生理生态,E-mail:phb9609@163.com
  • 基金资助:
    国家自然科学基金项目(31760118); 云南省中青年学术和技术带头人后备人才项目(2019HB013)

Effects of light intensity on behavior, learning memory and oxidative stress of Tupaia belangeri

  1. 1. Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan Province Higher Institutes College, School of Life Sciences, Yunnan Normal University,Kunming 650500, China; 2. Yunnan College of Business Management, Kunming 650106, China
  • Online:2022-02-18 Published:2022-02-15

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摘要：

为探讨不同光照强度对中缅树鼩行为变化、学习记忆能力和氧化应激的影响,分别于光照628、1 256 和2 512 lx 条件下测定体重、摄食量、Y 型迷宫正确反应率和行为变化,并测定丙二醛(Malondialdehyde,MDA)浓度、超氧化物歧化酶(Superoxide dismutase,SOD)、乙酰胆碱酯酶(Acetylcholinesterase,AChE)和一氧化氮合酶(Nitric oxide synthase,NOS)浓度,以及SOD、AChE 和NOS 的活性。结果表明,第28 天时3 组之间体重差异显著,摄食量的变化趋势和体重变化相似。强光照明显降低中缅树鼩的取食行为,增加休息行为。第28 天时,不同光照强度组之间识别正确率差异极显著。不同光照强度对MDA、SOD 浓度和SOD 活性影响差异极显著,对NOS 和AChE 活性影响极显著。结果说明,强光照可以降低中缅树鼩的体重和取食行为,增加休息行为,可诱发中缅树鼩的认知障碍和脑组织内不同程度的氧化损伤。

关键词: 中缅树鼩, 光照强度, 行为, 记忆, 氧化应激

Abstract:

To investigate the effects of different light intensities on the behavior changes, learning memory ability and oxidative stress inTupaia belangeri, body mass, food intake, behavior and correct response rate in Y-maze, concentrations of malondialdehyde (MDA),superoxide dismutase (SOD), acetylcholinesterase (AChE) and nitric oxide synthase (NOS), as well as activities of SOD, AChE and NOS were measured in T. belangeri, which were continuously exposed to 628, 1 256 and 2 512lx. The results showed that body mass had significant differences among three groups, changes of food intake were similar to body mass in T. belangeri. Higher light intensity reduced feeding behavior and increased resting behavior significantly. On day 28, there was a significant difference in recognition accuracy among different light intensity groups. Different light intensity had significant effects on the concentrations of MDA, concentration and activity of SOD, and the activities of NOS and AChE. All of the results indicated that higher light intensity reduced body mass and feeding behavior, increased rest behavior, and induced cognitive impairment and caused different degrees of oxidative damage in T. belangeri.


Key words: Tupaia belangeri, light intensity, behavior, memory, oxidative stress

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