Abstract: To investigate the formation and depolymerization of stress granules in zebrafish embryonic fibroblasts cell (ZF4) under cold stress, 18 ℃, 13 ℃ and 10 ℃ were selected as the cold stress temperature, and the low temperature treatment was carried out at different times； and then TIAL1 and G3BP1 (marker of stress granules) were used to conduct cellular immunofluorescence experiments and RT-qPCR experiments； finally, the temperature and time of stress recovery were selected to investigate the depolymerization of stress granules (SG). The results showed that under cold stress at different temperatures, stress granules were formed at different time in ZF4 cells， and the stress granules had different sizes, with the increase of low temperature treatment time. The stress granules of ZF4 cells were formed when treated at 18 ℃ for 24 h, 13 ℃ for 4 h and 10 ℃ for 1 h. As the temperature decreased, the rate of formation of stress granules also accelerated. The formation of stress granules is manifested by a gradual increase in stress granules and an increase in the size of the diameter. When ZF4 cells were treated at 13 ℃ for 4 h and then the temperature was restored to the optimum growth temperature (28 ℃), it was found that the stress granules depolymerized rapidly in about ten minutes. Depolymerization of stress granules is manifested by the rapid dissolution of stress granules. This paper firstly found that fish cells form stress granules at low temperature, and the main components are similar to mammalian cells, indicating that stress granules are the general response mechanism of animal cells to cold stress.

Key words: ZF4 cells, cold stress, stress granules, formation, depolymerization