Abstract: The purpose of this research is to compare the extraction and purification of nucleic acid, including bacterial genomic DNA, plasmid DNA and bacterial total RNA by using the method of magnetic beads and kit and Trizol methods. The results showed that compared with the kit method and Trizol method, the magnetic bead method was more efficient in the extraction of bacterial genomic DNA and RNA, PCR product detection and purification. However, the efficiency of PCR product recovery and plasmid DNA extraction was lower than that of the kit method, and plasmid enzyme cutting effect was not different from that of the kit method. The results showed that the nanometer magnetic bead method is better than the kit and Trizol methods in nucleic acid extraction and purification.

Key words: rapid nucleic acid extraction, nucleic acid purification, magnetic bead method, kit method