建立表达犬NECTIN4的牛源细胞株用于培养犬瘟热病毒

doi:10.3969/j.issn.2095-1736.2019.02.007

Establishment of bovine cell line expressing canine NECTIN4 for canine distemper virus culture

1. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122;2. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology,Jiangnan University, Wuxi 214122; 3. Oncology Department, Affiliated Hospital of Jiangnan University, Wuxi 214062, China

Online:2019-04-18 Published:2019-04-18

Abstract

Abstract: Nectin cell adhesion molecule 4 (NECTIN4) is one of the receptors for canine distemper virus (CDV). In this study, a Madin-Darby Bovine Kidney (MDBK) cell line expressing canine NECTIN4, designated MDBK-N4, was established for CDV culture. We found that MDBK-N4 cell line could multiply the virus and it was caused typical cytopathic effect (CPE) after CDV inoculation, which demonstrated that transfection with viral receptor complementary DNA may enables a non-susceptible cell line to bind virus, support virus replication and develop CPE. Viral receptors expression provides a new way to develop a cell line for vaccine production in industry.

Key words: canine distemper virus, NECTIN4, MDBK, receptor

CLC Number:

Q291

YANG Si-ming, CAI Dong-yan, SUN Man-man, CHEN Xiao, DAI Xiao-feng, BAI Zhong-hu. Establishment of bovine cell line expressing canine NECTIN4 for canine distemper virus culture[J]. , doi: 10.3969/j.issn.2095-1736.2019.02.007.

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Establishment of bovine cell line expressing canine NECTIN4 for canine distemper virus culture

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