Abstract: An experiment was conducted to study the individual or joint toxicity of aflatoxin B1 (AFB1) and deoxynivalenol (DON) in mice. Ninety-six Kunming male mice (21-day-old) were randomly divided into four groups, three replicates per group and eight per replicate. All mice were fed with the same feedstuff during the experiment. Mice in group Ⅰ were gavaged with the physiological saline every day; the mice in group Ⅱ were gavaged with 200 μg·kg-1 AFB1; the mice in group Ⅲ were gavaged with 500 μg·kg-1 DON and the mice in group Ⅳ were gavaged with 200 μg·kg-1 AFB1 + 500 μg·kg-1.The trial was conducted for 30 days. On the 15th and 30th day, four mice from each replicate were selected randomly and blood samples were taken from the eyeball for measuring serum biochemical indexes. Meanwhile, the selected mice were slaughtered and the liver and spleen were taken for calculating the organ relative indexes, and the pathologic changes of liver tissues were observed. The results showed that, compared with the control group, the relative organ indexes of liver and spleen, serum AST activities and MDA contents of mice in Group Ⅳ were significantly increased , the ALB and TP contents were significantly decreased on the 15th day. Compared with Group Ⅱ and Group Ⅲ, the relative organ indexes of liver and spleen, serum AST activities and MDA contents of mice in Group Ⅳ were significantly increased. On the 30th day, compared with the control group, weight gain of mice in Group Ⅱ, Ⅲ, and Ⅳ were significantly decreased; serum ALT, ALP, r-GT activities of mice in Group Ⅳ were significantly increased, but SOD activities, and TP, ALB contents were significantly decreased. Compared with Group Ⅱ and Group Ⅲ, serum ALP and r-GT activities, and MDA contents of mice in Group Ⅳ were significantly increased, while serum SOD activities were significantly decreased. These experimental results suggest that AFB1 and DON can decrease the weight gain, liver and spleen relative indexes, and alter the biochemical indexes; the joint toxic effects of AFB1and DON are higher than any of the individual toxicology.

Key words: aflatoxin B1, deoxynivalenol, joint toxicity, biochemical index, mice