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Expression analysis of JNK1 in different developmental phases of gonad in goldfish Carassius auratus

  

  1. Laboratory of Basic Biology, Hunan First Normal University, Changsha 410205, China
  • Online:2017-08-18 Published:2017-08-18

Abstract: The objective of this study was to explore the correlation of JNK1 gene in the process of gonad development of Goldfish (Carassius auratus). The expression of JNK1 was studied and analyzed in different developmental phases of gonad by using quantitative real-time PCR and Western blotting. It was found that gonadosomatic index (GSI) of males and females showed significant seasonal variation with the development process of gonad and was the maximium during the breeding period (P <0.05). The qPCR analysis revealed that the expression of JNK1 firstly increased and then decreased at the development phases of ovary, reached the peak level at maturity phase (P <0.05),4.0 times higher before sexual maturity, 1.4 times higher degradation phase and 2.1 times higher at the rest phase compared with that at maturity phase.In the testis, the expression of JNK1 was found to be trace enhanced compared with before sexual maturity phase, however, the expression changes had no significant difference in the whole development process. Western blotting analysis showed that expression tendency of JNK1 protein was in line with that of JNK1 mRNA. Together, these results suggest that JNK1 plays an important role in the processes of the development of ovary and promoting the maturation of oocytes in fish.

Key words: Carassius auratus; JNK1 gene; gonadal development, gonadosomatic index