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The study of atrazine degraded by endophytic fungus from sugarcane

  

  1. 1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University,Nanning 530004; 2. College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004;3. Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
  • Online:2017-02-18 Published:2017-02-18

Abstract: An endophytic fungus strain of degrading atrazine was isolated from the roots of sugarcane in Guangxi farmland which has been sprayed by atrazine for a long time. It was preliminarily identified as Fusarium fujikuroi according to its morphological characteristics and the analysis of its 18S rRNA and ITS gene sequences. The degradation characteristics were also investigated. The results showed that the strain could use atrazine as sole nitrogen source and had the best degradation property under those conditions: pH 7-8, temperature 28℃, atrazine primary concentration 25 mg/L. The degradation rate could reach 49.6% after 10 days. The degradation products were determined by ultra performance liquid chromatography-mass spectrometry (UPLC-MS). The results showed that the degradation product was cyanuric acid.

Key words: atrazine, biodegradation, endophyte, degrading characteristics