Abstract: This experiment was implemented to investigate the differences of the metabolites in blueberry fruits after the application ofBacillus tequilensisfermentation. The resistance mechanism of postharvest blueberry fruits treated byB.tequilensisfermentation was explored and the theoretical references for the application ofB.tequilensisin blueberry fruits storage and preservation were provided. Blueberry fruits were treated withB.tequilensisfermentation, the lesion diameters were measured. Subsequently, non-targeted metabolomics techniques were used to analyze the metabolites of blueberry fruits treated withB.tequilensisfermentation. Then, the content of phenolic compounds and flavonoids in blueberry fruit was determined. The result showed that blueberry fruit lesion diameters were significantly reduced after treated withB.tequilensisfermentation. Based on non-targeted metabolomics techniques, 77 pathways were annotated, and a total of 27 significant differential metabolites were enriched in the 20 metabolic pathways with the highest correlation in the group after treated withB.tequilensisfermentation. Among them, six pathways with significant influence were listed as follows: biosynthesis of phenylpropanoids, flavone and flavonol biosynthesis, biosynthesis of siderophore group nonribosomal peptides, flavonoid biosynthesis, apoptosis and C5-Branched dibasic acid metabolism. And the content of phenolic compounds and flavonoids in blueberry fruit was higher than that in the control treatment, which was consistent with the results of metabolomics analysis. After the treated withB.tequilensisfermentation, secondary metabolites accumulated in blueberry fruit, which in turn increased the disease resistance of blueberry fruits.

Key words: Bacillus tequilensis, blueberry fruit, metabolomics, secondary metabolites