Journal of Biology ›› 2024, Vol. 41 ›› Issue (4): 78-.doi: 10.3969/j.issn.2095-1736.2024.04.078

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Study of pollen germination vigor and morphology in Actinidia deliciosa

HE Liqin1,2, WANG Lihua1,2, ZHENG Xiaoqin3, ZHANG Qian1,2, LIANG Shuang3 , LI Qiaohong1,2   

  1. 1. Sichuan Provincial Academy of Natural Resource Sciences, Chengdu 610015, China;
    2. China-New Zealand Belt and Road Joint Laboratory on Kiwifruit, Chengdu 610015, China;
    3. College of Food and Bioengineering, Chengdu University, Chengdu 610106, China
  • Online:2024-08-18 Published:2024-08-14

Abstract: In order to investigate the viability of kiwifruit pollen, and to study the morphological changes before and after pollen germination, 15 samples ofActinidia deliciosapollens were used in this experiment. Anin vitroculture method was used to determine pollen germination viability, and scanning electron microscopy was used to observe the morphology of five female pollens and ten male pollens before and after germination. The results showed that the germination rate of pollens from male plants varied considerably. None of the five female pollens germinated. Among the 10 male pollen samples No. 8 had the highest germination rate of 79.27% and No. 9 had the lowest germination rate of 22.85%. The polar facial view of the 15 samples did not change before and afterex vivoincubation. These samples were all trilobate circles. However, there was a significant difference in their equatorial view. The pollen morphology did not change before and after the female plants were cultured in isolation, and they were all spheroidal. The pollens of the male plant were perprolate or prolate before germination, and after germination in culturein vitro, all 10 samples were sub-spherical, with the polar axis length becoming almost smaller, the equatorial axis length becoming larger, the P/E ratio becoming smaller, and the width of the germination groove becoming larger. The differences in the values of the morphological indexes between the samples were small.

Key words: kiwifruit, pollen;in vitroincubation, germination vigor, morphology

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