Abstract: A bacterial strain producing high-activity and high-temperature alkaline pectinase was obtained from the soil and was named as AP-1. The characteristics and the separation and purification of the pectinase produced by AP-1 were preliminarily studied. The result of the study on pectinase activity showed that the optimal temperature and pH of the pectinase were 70℃ and 7.5, respectively. The experiment of stability showed that the enzyme had good resistance to alkali and was activated remarkably by Mg2+ under the same conditions. The separation and purification showed that the purification effect was better when the enzyme was treated by 80% saturation of ammonium sulfate solution than other, but the purification effect was the best when the enzyme was treated by ethanol.

Key words: alkaline pectinase, enzymatic properties, separation and purification