Abstract: 16S rRNA high-throughput sequencing was used to analyze the intestinal microbial structure and characteristics of Anserfabalis in Yangetou (SLY) and Chi’an (SLC) habitats of Shengjin Lake. The results showed that there was a significant difference in the abundance of intestinal microbiome flora between soybean geese in the two habitats of SLY and SLC in Shengjin Lake (P< 0.05). Firmicutes and Proteobacteria were the dominant bacteria in SLY, and Firmicutes and cyanobacteria were the dominant bacteria in SLC. Lactobacillus (39.5%) was the dominant bacteria in the intestinal flora of Anserfabalis in Shengjin Lake SLY habitat, while norank was the dominant bacteria in the intestinal flora of Anserfabalis in Shengjin Lake and SLC habitat norank_ f__ norank_ o__ Chloroplast (35.3%) and Lactobacillus (19.2%). Based on Bray_ NMDS analysis of Curtis distance matrix showed that the flora in the two habitats showed obvious taxonomic groups. The correlation of intestinal flora network of Anserfabalis in SLC habitat was closer and more complex than that in SLY. In addition, Picrust’s function prediction showed that the cog function annotation results of the intestinal microbiome of Anserfabalis in SLY and SLC habitats mainly included function prediction function, amino acid transport and metabolic replication function, recombination and repair function, signal transduction mechanism function, energy production and conversion function, etc. The results showed that there were significant differences in the composition of intestinal flora of Anserfabalis in Shengjin Lake amphibious habitat at the beginning of winter, and showed unique flora characteristics. The results provided basic data and research direction for the follow-up research and protection of migratory birds in the whole overwintering period.

Key words: Shengjin Lake, different habitat, 16S rRNA amplicon sequencing, intestinal flora