Abstract: In order to meet the needs of cultivating high-quality talents in biological science major, based on modern information technology, the independent campus SPOC of biochemistry experiment was built on the public MOOC platform, and the online and offline mixed teaching modes were explored and practiced. The teaching process of each experimental project was divided into four main stages: pre-class preparation, online teaching, offline teaching and after-class summary. Online teaching mainly completed the teaching contents of experimental principle, reagent preparation, instrument use and demonstration of experimental operation process, while offline teaching was mainly based on students’ hands-on operation. Online teaching was well prepared for offline teaching, which not only improved the efficiency of offline teaching, broadens students’ vision by experimental items of expanded, improved students’ comprehensive quality through ideological and political education, but also improved the objectivity and accuracy of curriculum assessment, increased the degree of grade differentiation through the combination of online assessment and offline assessment, improved students’ interest and autonomous learning ability, realized the cooperative cultivation of knowledge, quality and ability, and provided a reference and guidance for the reform of online and offline mixed teaching mode in other experimental courses.

Key words: biochemistry experiment, mixed teaching model, bioscience majors