Abstract: In order to understand the bacterial community structure in the farmland soil of highland barley planting in Lhasa, and to clarify the influence of different growing periods and different locations of highland barley on the bacterial diversity, the community structure and diversity of bacteria in highland barley cultivation soil were analyzed by high-throughput sequencing technology with the highland barley cultivation soil as the experimental material. Result showed that 37 265-40 008 effective sequences and 4094-4730 OTU were obtained from each sample of highland barley soil microbial sequencing in this study. According to the rarefaction curve and sequencing depth index, the result of the sequencing was more comprehensive covering the soil microorganism community. Shannon index and OTU number of each sample in different periods showed Group 1 (March 25, sowing early) 

Key words: Lhasa, highland barley, farmland soil, bacterial diversity, illumina miseq sequencing