生物学杂志

生物学杂志 ›› 2019, Vol. 36 ›› Issue (5): 78-.doi: 10.3969/j.issn.2095-1736.2019.05.078

• 综述与专论 • 上一篇    下一篇

嗜热酯酶的研究进展

  

  1. 1.江苏大学 生命科学研究院， 镇江 212000; 2.江苏中兴药业有限公司， 镇江 212000
  • 出版日期:2019-10-18 发布日期:2019-10-11
  • 通讯作者: 陈华友，博士，教授，研究方向为应用微生物学，E-mail: hyc@ujs.edu.cn
  • 作者简介:李婷婷,硕士研究生,研究方向为应用微生物学,E-mail: m18851400132@163.com
  • 基金资助:
    江苏省重点研发计划（现代农业）（BE2017355）；江苏省农业科技自主创新资金项目CX（17）3044

Research progress of thermophilic esterase

  1. 1. College of Life Science, Jiangsu University, Zhenjiang 212000; 
    2. Jiangsu Zhongxing Pharmaceutical Co., Ltd, Zhenjiang 212000， China
  • Online:2019-10-18 Published:2019-10-11

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摘要： 酯酶（Esterases, EC 3.1.1.X）是一种催化酯键水解与合成的酶的总称，在生物催化领域具有广泛的应用。在工业生产与应用中，来自嗜温微生物的酯酶在诸如高温、高压、有机试剂存在的环境中很不稳定，极易丧失活性；而来自嗜热微生物的嗜热酯酶由于其来源特殊，在耐热、耐有机试剂以及催化速率等方面拥有特殊优势，近些年成为生物催化领域研究的焦点。首先分析了嗜热酯酶的来源，阐述了嗜热酯酶的耐热机制，讨论了其固定化方法，最后对其主要应用以及研究进展进行了总结，并指出当下嗜热酯酶研究的不足及未来的发展趋势。

关键词: font-size:medium, ">嗜热酯酶；酯酶；固定化

Abstract: Esterases (Esterases, EC 3.1.1.X) is a general term for enzymes that catalyze the hydrolysis and synthesis of ester bonds and has been widely used in the field of biocatalysis. In industrial production and application, esterases from the mesophilic microorganisms are unstable and easily lose their activity in environments, such as high temperature, high pressure and organic reagents however, thermophilic esterase, because of its origin, has unique advantages in heat resistance, organic reagents and catalytic rate and so on. In recent years, thermophilic esterase has become the focus of biological catalysis research. This paper firstly analyzed the various sources of thermophilic esterase, expounded the heat resistant mechanisms of thermophilic esterase, discussed its immobilization methods, and then the main application and research progress were summarized, and the shortage in the contemporary study of thermophilic esterase and the development trend of the future was pointed out .

Key words: thermophilic esterase, esterase, immobilization

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