生物学杂志

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蛛丝蛋白PySp用于静电纺纤维膜的研究

  

  1. 东华大学 生物科学与技术研究所, 上海 201620
  • 出版日期:2019-02-18 发布日期:2019-02-18
  • 通讯作者: 孟清,博士生导师, 研究方向为编码蜘蛛丝蛋白质基因的克隆、表达、结构与功能、成丝机理及仿生研究,E-mail: mengqing@dhu.edu.cn
  • 作者简介:沈庆春,硕士研究生,主要从事人造蛛丝的应用研究,E-mail: qingchunshen@foxmail.com
  • 基金资助:
    国家自然科学基金面上项目(No. 31570721);上海市科委攻关项目(No. 14521100700);上海市科委国际合作项目(No. 14520720200)

Study on spidroin PySp using electrospinning as fiber membrane

  1. Institute of Biological Science and Biotechnology, Donghua University, Shanghai 201620, China
  • Online:2019-02-18 Published:2019-02-18

摘要: 蛛丝纤维具有强度高、柔韧性及生物相容性好等材料学特性,因而蛛丝蛋白在人造血管、神经导管及药物载体等方面有潜在巨大价值。将本课题组前期得到的大腹圆蛛梨状腺丝的一个重复区(Rp)进行原核表达,得到蛛丝蛋白作为实验材料。通过探究静电纺丝条件,将该蛋白纺成纳米纤维膜。SEM观察显示该膜形成的纤维均匀一致;ATR-FTIR测试表明,经过75%乙醇交联的静电纺纳米纤维的二级结构主要为β折叠;接触角证明,纳米纤维膜是亲水的。为蛛丝蛋白应用于组织工程提供了一种良好的纤维材料,对蛛丝蛋白的进一步应用奠定了基础。

关键词: 蛛丝蛋白, 静电纺丝, 纤维膜, 性能表征

Abstract: Recombinant spider silk, as a kind of biological material with good performance, owns a great biocompatibility, meanwhile, there is great potential application value in the field of biomedical. Spider silk has potential application value in blood vessels, nerve conduit and drug carriers, but still lacks research in the tissue engineering. In this study, spidroin PySp was expressed in E.coli as the material. By investigating the electrospinning conditions, fiber membrane was obtained at a suitable spinning voltage and other conditions. SEM displayed that fiber membrane is uniform. The ATR-FTIR graph showed that the secondary structure of the electrospun fibers cross-linked by 75% ethanol is mainly β-folded. The contact angle revealed that the fiber membranes are hydrophilic. This study has provided a type of good fiber material for the application of spider silk protein in tissue engineering and laid a foundation for the further application of spider silk.

Key words: spidroin, electrospinning, fiber membrane, performance characterization

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