生物学杂志

生物学杂志

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卡介苗培养滤出液中O-甘露糖基化蛋白的确定和解析

  

  1. 大连医科大学 微生物学教研室, 大连 116044
  • 出版日期:2019-08-18 发布日期:2019-08-18
  • 通讯作者: 孙文长,副教授,从事病原微生物研究, E-mail: 2981264350@qq.com
  • 作者简介:杨淑凤，博士,讲师,从事分枝杆菌糖蛋白功能和卡介苗免疫研究，E-mail:shufengyang78@163.com；邓国英，共同第一作者,博士,讲师,从事分枝杆菌免疫机制研究, E-mail: dhgxy2000@163.com
  • 基金资助:
    大连医科大学基础医学院助飞基金（2016年第一批）

Identification and analysis of O-mannosylated proteins from Bacillus Calmette-Guérin culture filter

  1. Department of Microbiology, Dalian Medical University, Dalian 116044, China
  • Online:2019-08-18 Published:2019-08-18

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摘要： 卡介苗作为预防结核病的唯一疫苗，不能提供全面的保护力，因此全面认识卡介苗的抗原非常关键。甘露糖复合物抗原是存在于分枝杆菌细胞壁上的一大类重要抗原，在细菌毒力以及宿主细胞相互作用等方面发挥作用，其中O-甘露糖基化蛋白在结核分枝杆菌和耻垢分枝杆菌中陆续有报道，但在卡介苗中研究很少。利用Con-A亲和层析技术提纯了卡介苗培养滤出液中的O-甘露糖基化蛋白，并经过ConA-免疫印迹再次确证，最后利用质谱技术进行了解析，共确定出7个O-甘露糖基化蛋白，并对功能和糖基化位点进行了初步预测。O-甘露糖基化蛋白的确定和解析将有助于对卡介苗免疫机制的认识。

关键词: 卡介苗, O-甘露糖基化蛋白, Con-A亲和层析

Abstract: As the only licensed vaccine to protect against tuberculosis, Bacillus Calmette-Guérin（BCG）can not provide complete protection，therefore, it is essential to fully understand the antigens of BCG. The mannose containing glycoconjugates, which are located on cell wall, play a role in mycobacterial virulence and interaction with host cell. O-mannosylated proteins were reported in Mycobacterium tuberculosis and Mycobacterium smegmatis, but seldom in BCG. In this article, the O-mannosylated proteins from Bacillus Calmette-Guérin culture filter were identified and analyzed by ConA-agarose affinity chromatography combined with MALDI mass spectrometer. There were 7 kinds of O-mannosylated proteins identified and the mannosylated positions of these 7 proteins were further predicted. The identification and analysis of O-mannosylated proteins will shed light on immune mechanism of BCG.

Key words: Bacillus Calmette-Guérin（BCG）, O-mannosylated protein, ConA affinity
     

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