生物学杂志

生物学杂志

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纳米氧化锌对HEK293细胞的毒性作用研究

  

  1. 苏州大学 基础医学与生物科学学院，江苏苏州 215123
  • 出版日期:2016-02-18 发布日期:2016-02-18
  • 通讯作者: 韩宏岩，副教授，研究方向为生物化学与分子生物学，E-mail：hhy2469@126.com。
  • 作者简介:陈玉莹，硕士研究生，研究方向为纳米毒理学，E-mail：yuying226@163.com；
  • 基金资助:
     
    国家自然科学基金项目（No.81071306）

 
Cytotoxicity of ZnO Nanoparticles on HEK293 cells

  1. School of Life Science，Soochow University，Suzhou 215123，China
  • Online:2016-02-18 Published:2016-02-18

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摘要：  

探讨纳米氧化锌浓度和尺寸效应对HEK293细胞的毒性作用以及其产生毒性的原因。分别用不同浓度的纳米氧化锌（15nm）和不同尺寸的纳米氧化锌：常规氧化锌（颗粒直径≤1μm）、15nm氧化锌、30nm氧化锌、90nm氧化锌处理HEK293细胞，48h后，MTT检测纳米氧化锌对HEK293细胞活性的影响，流式细胞仪检测纳米氧化锌对HEK293细胞凋亡的影响，ROS检测试剂盒检测纳米氧化锌对HEK293细胞中ROS含量的影响。结果显示当15nm氧化锌浓度小于2.0μg/mL时，HEK293细胞活性、凋亡率和ROS水平与对照组相比没有明显差别；但当纳米氧化锌的浓度大于4.0μg/mL时，HEK293细胞活性呈明显下降趋势，细胞凋亡率和ROS含量呈明显的上升趋势且在同一浓度下，纳米氧化锌尺寸越小，HEK293细胞活性越低，细胞凋亡率和ROS含量越高。该结果表明纳米氧化锌对HEK293细胞的毒性作用呈明显的剂量效应和尺寸效应，纳米氧化锌诱导HEK293细胞产生的大量活性氧（ROS）可能是导致其产生细胞毒性的主要原因之一。

关键词: 纳米氧化锌, HEK293细胞, 细胞毒性, 细胞凋亡, ROS

Abstract:  

 This experiment was to explore the effects of concentration and size of ZnO Nanoparticles (ZnO NPs) on HEK293 cells and research its mechanism of toxicity. In this experiment, we used three different sizes ZnO NPs which include 15nm-sized ZnO NPs, 30 nm-sized ZnO NPs, 90nm-sized ZnO NPs and normal ZnO to treat HEK293 cells. After 48 hours, we detected the cell proliferation by MTT test, then we made a cell cytometry to investigate the rate of apoptosis. Last, we analyzed the change of ROS level by kit. There was no noticeable changes on HEK293 cells treated with 15nm-sized ZnO NPs below 2.0μg/mL concentration. While above 4.0μg/mL, the proliferation of HEK293 cells was reduced significantly. However therate of apoptosis and the level of ROS increased obviously. With the decrease of the size of ZnO NPs, the rate of apoptosis and the level of ROS increased, but the proliferation of cell gradually reduced in HEK293 cells. In this experiment, the result suggest that cytotoxicity of ZnO NPs on HEK293 cells be related to its concentration and size. Increasing of ROS induced by ZnO NPs may be one important element of cytotoxicity.