生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (3): 28-.doi: 10.3969/j.issn.2095-1736.2023.03.028

• 研究报告 • 上一篇    下一篇

兰州百合内生放线菌Streptomyces sp. LRE-541次级代谢物抗胃癌活性研究

陈　彬1,2， 姜　侃1,2， 郭晔红1,2， 马爱爱3   

  1. 1. 甘肃农业大学 农学院， 兰州 730070； 2. 甘肃省干旱生境作物学重点实验室，
    兰州 730070； 3. 西北民族大学 生命科学与工程学院， 兰州 730030
  • 出版日期:2023-06-18 发布日期:2023-06-19
  • 通讯作者: 姜侃，博士，副教授，研究方向为天然药物化学，E-mail:jiangk19@126.com；郭晔红，博士，教授，研究方向为药用植物栽培，E-mail:guoyh@gsau.edu.cn;姜保和郭晔红为共同通信作者
  • 作者简介:陈彬，硕士研究生，研究方向为天然药物化学，E-mail:2279507859@qq.com

Antigastric cancer activity of Streptomyces sp. LRE-541 secondary metabolite from Lilium davidii var. unicolor Cotton

CHEN Bin1,2, JIANG Kan1,2, GUO Yehong1,2, MA Aiai3   

  1. 1. College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China;
    2. Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou 730070, China;
    3. Life Science and Engineering College, Northwest University for Nationalities, Lanzhou 730030, China
  • Online:2023-06-18 Published:2023-06-19

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摘要： 研究兰州百合(Lilium davidii var. unicolor Cotton)根部内生放线菌Streptomyces sp. LRE-541的次级代谢产物及抗胃癌活性，利用高效液相色谱法(HPLC)、核磁共振(NMR)等方法对该菌株次级代谢产物进行分离纯化及鉴定；以胃癌细胞HGC-803为研究对象，探讨代谢产物对细胞增殖细胞凋亡和细胞迁移能力的影响。结果从其发酵液中获得两个蒽醌类化合物，鉴定为4-Deoxy-ε-pyrromycinone (1)和epsilon-pyrromycinone (2)。化合物1和化合物2对胃癌细胞HGC-803的IC50分别为(25.24±1.23) μg/mL和(17.29±2.28) μg/mL；在相同处理下，与空白对照组相比，15、25 μg/mL的化合物1组和10、20 μg/mL的化合物2组癌细胞增殖率、细胞凋亡率和细胞划痕愈合率明显下降，差异具有统计学意义(P＜0.05或P＜0.01)。表明这两种化合物都可抑制胃癌HGC-803细胞增殖和迁移能力，促进细胞凋亡，有成为抗胃癌药物或前体药物的潜力。

关键词: 植物内生放线菌, 蒽醌类化合物, 细胞增殖, 细胞凋亡, 细胞迁移

Abstract: n order to study the secondary metabolites of Streptomyces sp. LRE-541 from the roots of Lilium davidii var. unicolor Cotton and its antigastric cancer activity, high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) and other methods were used to isolate, purify and identify the secondary metabolites. The effects of metabolites on cell proliferation, apoptosis and migration of gastric cancer cell HGC-803 were investigated. Two anthraquinones were identified as 4-deoxy-ε-pyrromycinone (1) and epsilon-pyrromycinone (2). The IC50 of compound 1 and compound 2 against gastric cancer cell of HGC-803 were (25.24±1.23) μg/mL and (17.29±2.28) μg/mL, respectively. Under the same treatment, compared with blank control group, the proliferation rate, apoptosis rate and scratch healing rate of cancer cells in 15 and 25 μg/mL of compound 1 and 10 and 20 μg/mL of compound 2 groups were significantly decreased, the differences were statistically significant (P<0.05 or P<0.01). These results indicated that the two compounds could inhibit the proliferation and migration of gastric cancer of HGC-803 cells and promote the apoptosis of gastric cancer cells, which might be potential as anti-gastric cancer drugs or precursors.

Key words: ndophytic actinomycetes, anthraquinones, cell proliferation, apoptosis, cell migration

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