生物学杂志

• 技术方法 • 上一篇    下一篇

雨生红球藻中虾青素萃取工艺优化

  

  1. 宁波大学 海洋学院, 宁波 315211
  • 出版日期:2017-02-18 发布日期:2017-02-18
  • 通讯作者: 徐年军, 研究员, 博士生导师, 研究方向为藻类生理生化, E-mail:xunianjun@nbu.edu.cn
  • 作者简介:徐煜, 硕士, 研究方向为藻类生物技术, E-mail:bioxuyu@163.com
  • 基金资助:
    国家自然科学基金(31572638);浙江省科技厅公益项目(2015C32021); 宁波市科技计划项目(2014C10023)

Optimization of extracting process of astaxanthin from Haematcoccus pluvialis

  1. School of Marine Sciences, Ningbo University, Ningbo 315211, China
  • Online:2017-02-18 Published:2017-02-18

摘要: 建立了雨生红球藻中虾青素的超声波提取和高效液相色谱检测方法, 优化了雨生红球藻虾青素萃取工艺各项参数。实验数据显示: 相对于其他有机溶剂, 最佳提取溶剂为丙酮, 以虾青素含量作为衡量标准, 各溶剂提取效率的顺序为: 丙酮>二氯甲烷/甲醇(1∶ 3, V/V)>甲醇>石油醚>氯仿。通过响应面实验, 对影响虾青素提取的各项因素实施了优化, 确定了超声波辅助提取虾青素的最佳工艺参数: 料液比1∶ 377, 超声时间5.19 min, 超声波功率为145 W, 虾青素含量的预测值为2.931%, 在优化条件下, 虾青素含量实际测定值为2.896%。在该条件下,雨生红球藻虾青素含量明显提高。

关键词: 雨生红球藻, 虾青素, 超声波提取法, 响应面, 高效液相色谱法

Abstract: This paper established a method for astaxanthin extracting by ultrasonic extraction and detection by high performance liquid chromatography(HPLC). The result showed that acetone was the optimal solvent among the selected solvents. The astaxanthin yield of Haematococcus pluvialis was used as measurement index, the sequence of extraction efficiency of different solvent was that acetone> methylene chloride: methanol(3∶ 1, V/V) > methanol > petroleum ether > chloroform. The mathematical model of ultrasound assisted astaxanthin extraction of H.pluvialis was established by response surface method. The optimal condition for extraction technique was that S/L 1∶ 377, ultrasonic time 5.19 min and ultrasonic power 145 W. Under the optimal condition, the predicted astaxanthin yield was 2.931%, and the measured value was 2.896%. It indicated that the adaptable of model was good. Compared with conventional reflux extraction methods, extraction efficiency of astaxanthin was improved significantly assisted by response surface method.

Key words: Haematococcus pluvialis, astaxanthin, ultrasound assisted extraction, response surface, HPLC