生物学杂志

生物学杂志

• 技术方法 • 上一篇    下一篇

基于免疫层析检测的河豚毒素快速样品前处理方法研究

  

  1. 上海海洋大学食品学院 上海水产品加工及贮藏工程技术研究中心 农业部水产品贮藏保鲜质量安全风险评估实验室(上海)， 上海 201306
  • 出版日期:2017-02-18 发布日期:2017-02-18
  • 通讯作者: 卢瑛，博士，副教授，研究方向为食品安全检测、纳米生物技术，E-mail：y-lu@shou.edu.cn
  • 作者简介:方国锋，硕士，从事食品营养与安全研究，E-mail：1249400576@qq.com
  • 基金资助:
    上海市科委项目（15320502100）；十二五”农村领域国家科技支撑计划项目（2015BAD17B02）；养殖暗纹东方鲀商品鱼净化和品质提升研究：青产学研2015-26

Rapid preparation technology for tetrodotoxin based on immunochromatographic detection

  1. College of Food Science and Technology, Shanghai Ocean University, Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China
  • Online:2017-02-18 Published:2017-02-18

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摘要： 为了建立适合河豚毒素免疫层析检测的前处理方法，采用超滤技术对前处理方法进行了优化，并对建立的磁性免疫层析试纸条进行了评价。研究结果显示，采用截留分子质量为10 ku的超滤处理，4000 r/min离心10 min的处理是最优的，可以降低溶液中的蛋白浓度，加标回收率在92.4%～126.0%之间，适合免疫层析检测。为河豚毒素的快速现场检测提供了前处理技术参考。

关键词: 河豚毒素, 前处理, 超滤, 免疫层析检测

Abstract: In order to establish a suitable pretreatment method for immunochromatographic detection for tetrodotoxin, in this study，the ultrafiltration technology was applied to optimize the pretreatment. The magnetic immunochromatographic test strip established by our laboratory was used for evaluation. The results showed that the optimal treatment of centrifugation at 4000 r/min for 10 min with a cut off molecular of 10 ku. This method was suitable for immunochromatographic assay with good recovery (92.4%-126.0%). Also, the concentration of protein in the solution reduced after pretreatment. This study provides technical support for rapid on-site test of tetrodotoxin.

Key words: tetrodotoxin, pretreatment, ultrafiltration, immunochromatographic detection