生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (2): 15-.doi: 10.3969/j.issn.2095-1736.2025.02.015

• 研究报告 • 上一篇    下一篇

稳态磁场联合二甲双胍改善2型糖尿病小鼠糖代谢和肠道菌群

程国峰1,2， 汪　滢1,2， 冯传林1,2， 廖钟财3， 卫　敏3， 方彦雯3， 张　欣1,2   

  1. 1. 中国科学院合肥物质科学研究院 强磁场科学中心， 合肥 230031；
    2. 中国科学技术大学 研究生院科学岛分院， 合肥 230026； 3. 和也智慧健康研究院， 安吉 313300
  • 出版日期:2025-04-18 发布日期:2025-04-14
  • 通讯作者: 张欣，博士，研究员，研究方向为磁生物学，E-mail:xinzhang@hmfl.ac.cn
  • 作者简介:程国峰，硕士研究生，研究方向为磁场对病理模型鼠的影响，E-mail:gfcheng@mail.ustc.edu.cn
  • 基金资助:
    国家自然科学基金项目（U21A20148）

Static magnetic field combined with metformin improves glucose metabolism and gut microbiota in T2D mice

CHENG Guofeng1,2, WANG Ying1,2, FENG Chuanlin1,2, LIAO Zhongcai3, WEI Min3,FANG Yanwen3, ZHANG Xin1,2   

  1. 1. High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031,
    China; 2. Science Island Branch of Graduate School, University of Science and Technology of China, Hefei 230026,
    China; 3. Heye Smart Health Research Institute, Anji 313300, China
  • Online:2025-04-18 Published:2025-04-14

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摘要： 二甲双胍因其良好的降糖疗效、安全性和对机体代谢的多种益处成为治疗2型糖尿病的一线药物。然而，临床上与用药剂量相关的不良反应也同样显著。因而，减小用药剂量并满足降糖效果成为优化临床用药的关键环节。同时，近年来有研究表明中等强度稳态磁场对糖尿病小鼠具有一定的改善效果，而磁场和降糖药物联用能否提升药物治疗效果仍然未知。将稳态磁场与二甲双胍联合使用来探究其对高脂饮食和链脲佐菌素诱导的2型糖尿病小鼠的影响，并对小鼠的空腹血糖、糖耐受、GLP-1以及胰岛素分泌量进行检测。结果发现，虽然磁场和较低剂量二甲双胍（150 mg/kg）联合使用的抗高血糖效果并未超过较高剂量二甲双胍（250 mg/kg），但优于磁场或低剂量二甲双胍单独使用。通过对小鼠肠道内容物进行16S rRNA基因测序分析发现，稳态磁场及联用二甲双胍后，小鼠肠道菌群中与糖尿病正相关的Ruminococcus菌和Adlercreutzia菌属丰度显著下降，并且Lactobacillus和Oscillospira等益生菌属的丰度经稳态磁场暴露后也有升高趋势。因此，研究表明此类型的磁场可以改善糖尿病小鼠的肠道菌群结构，并提高低剂量二甲双胍的降血糖效果，这可能为糖尿病的治疗提供一种易于实现、安全有效的潜在联合新策略。

关键词: 糖尿病, 稳态磁场, 二甲双胍, 肠道菌群, 联合治疗

Abstract: Metformin is a first-line treatment for type 2 diabetes (T2D) due to its excellent blood glucose-lowering effects, safety, and multiple metabolic benefits. However, significant side effects related to dosages are also observed in clinical practice. Therefore, reducing the dosage while achieving satisfactory glycemic control becomes a crucial step in optimizing clinical treatment. Recent studies have shown that static magnetic fields (SMFs) have certain ameliorative effects on diabetic mice, but the combinational effects of SMFs and hypoglycemic agents are still unknown. In this study, the effects on high-fat diet and streptozotocin-induced T2D mice by combining a downward, moderate-intensity SMF with metformin were investigated. The fasting blood glucose, glucose tolerance, GLP-1 levels, and insulin secretion were all assessed. The results revealed that although the antihyperglycemic effect of SMF combined with lower-dose metformin (150 mg/kg) was not greater than that of higher-dose metformin (250 mg/kg), it was superior to SMF or low-dose metformin alone. Furthermore, 16S rRNA gene sequencing analysis of intestinal contents revealed that the abundance ofRuminococcusandAdlercreutzia, which were positively related to diabetes, were significantly decreased by the combinational treatment of SMF and metformin. The abundance of probiotics such asLactobacillusandOscillospirawere also increased after SMF exposure. Therefore, this study showed that this type of SMF could improve the intestinal microflora of diabetic mice and enhance the hypoglycemic effect of low-dose metformin, which could potentially provide a new feasible, safe and effective combination strategy for the treatment of diabetes

Key words: diabetes, static magnetic fields, metformin, gut microbiota, combined treatment

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