关键词: 固定化酶, 纳米花, 蔗糖酶, 酶学性质, 酶活力

Abstract: Sucrase (Sucrase, EC 3.2.1.26) has high economic value in industry, and can be used to decompose sucrose to produce fructose and glucose. Nowadays, there are problems such as single sucrase fructose production method, not easy to maintain vitality and difficult to reuse. The nano-flower immobilized enzyme of Mn3(PO4)2/sucrase \[hereinafter referred to as Sucrase@Mn3(PO4)2\] was prepared by co-precipitation method, and the preparation conditions were optimized. The morphology and structure of the immobilized enzyme were characterized and the kinetic characteristics of the immobilized enzyme were analyzed. The results showed that the nano-flower particles of Sucrase@Mn3(PO4)2were uniform in size, dispersed, daisy-shaped and blooming. The internal crystal structure of nano-flower immobilized enzyme was complete. The immobilized enzyme had obvious EDS characteristic peaks of Mn2+, PO3-4and enzyme protein, and had obvious FT-IR absorption characteristics of enzyme protein and phosphoric acid. It showed good temperature stability and acid-base stability, and maintained its original activity of 40.38% after repeated use for 7 times and 40% when stored at 4 ℃ for 28 days.

Key words: immobilized enzyme, nano-flower, sucrase, enzymatic properties, enzyme activity