生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (6): 13-.doi: 10.3969/j.issn.2095-1736.2025.06.013

• 研究报告 • 上一篇    下一篇

黏质沙雷菌ZH-5产漆酶特性与全基因组测序分析

谭福民， 潘　莹， 王颖阔， 梁鑫鑫， 范恩国， 高翠娟   

  1. 临沂大学 生命科学学院， 临沂 276000
  • 出版日期:2025-12-18 发布日期:2025-12-19
  • 通讯作者: 高翠娟，博士，副教授，研究方向为微生物代谢与发酵，E-mail：gaocuijuan@lyu.edu.cn
  • 作者简介:谭福民，硕士在读，研究方向为微生物代谢与发酵，E-mail：tanfumin@lyu.edu.cn
  • 基金资助:
    国家自然科学基金项目（31700074）；临沂大学科技基金项目（LD-2023-KJ2-001）

Characterization of laccase production and complete genome sequencing in Serratia marcescens ZH-5

TAN Fumin, PAN Ying, WANG Yingkuo, LIANG Xinxin, FAN Enguo, GAO Cuijuan   

  1. College of Life Sciences, Linyi University, Linyi 276000, China
  • Online:2025-12-18 Published:2025-12-19

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摘要： 采用平板显色法从土壤中分离到一株产漆酶的微生物，结合16S rDNA和生理生化特征鉴定为黏质沙雷菌（Serratia marcescens），命名为ZH-5。基因组测序结果表明，菌株ZH-5基因组大小为5055904 bp，GC含量占比为59.55%，含有4643个基因，基因总长度为4423485 bp，占基因组的87.49%，基因组中含有漆酶基因（GE001633）和数条异源生物质降解途径。在单因素和酶促反应体系优化的基础上，结合碳氮源正交试验优化培养基组分，ZH-5菌株培养6 d后在含1 mmol/L Cu2+，蛋白胨20 g/L，酵母粉10 g/L，甘油20 g/L的改良LB液体培养基中产生的漆酶活力达到（24.68±0.76） U/mL，较优化前提高34.74倍。此外，对ZH-5菌株进行了菌体生长特性测定，结果发现，ZH-5具有较好的耐酸、耐盐特性和较广的生长温度。通过对ZH-5的全基因组测序和基因功能分析，充分表征了漆酶的活性，为后续开展基于ZH-5漆酶的应用研究奠定理论基础。

关键词: 菌株分离, 黏质沙雷菌, 漆酶, 酶活力, 全基因组测序

Abstract: In this study, the microbial strain designated as ZH-5 and capable of producting laccase was isolated from soil by plate chromogenic method and identified asSerratia marcescensby 16S rDNA and physiological and biochemical characteristics. Genome sequencing results showed that the genome size of strain ZH-5 was 5055904 bp, with GC content accounted for 59.55%, 4643 genes, and a total coding length of 4423485 bp, accounting for 87.49% of the genome. The genome contained a laccase gene (GE001633) and several heterologous biomass-degradation pathways. The enzyme reaction system was optimized firstly. The medium components of carbon and nitrogen sources were optimized further through single-factor optimization and orthogonal experiments. Based on single-factor and enzyme-catalyzed reaction system optimization, the medium composition was optimized through orthogonal experiments combining carbon and nitrogen sources. After 6 days of cultivation, the ZH-5 strain produced laccase activity of (24.68±0.76) U/mL in modified LB liquid medium containing 1 mmol/L Cu2+, 20 g/L peptone, 10 g/L yeast powder, and 20 g/L glycerol, representing a 34.74-fold increase compared to pre-optimization levels. In addition, the growth characteristics of ZH-5 were measured. The results showed that ZH-5 had better acid and salt tolerance and a broader temperature range for growth. Through the whole genome sequencing and gene function analysis of ZH-5, the activity of laccase was fully characterized, providing a theoretical basis for the subsequent application research based on ZH-5 laccase.

Key words: strain isolation;Serratia marcescens, laccase, enzyme activity, genome sequencing

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