生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (5): 35-.doi: 10.3969/j.issn.2095-1736.2023.05.035

• 研究报告 • 上一篇    下一篇

油茶炭疽病发生与丛枝菌根真菌（AMF）关系研究

杨娅琳1， 武自强1， 刘　丽1， 张东华1， 马焕成2， 伍建榕1   

  1. 1. 西南林业大学 生物多样性保护学院 云南省高校森林灾害预警控制重点实验室， 昆明 650224；
    2. 西南林业大学 林学院 西南地区生物多样性保育国家林业局重点实验室， 昆明 650224
  • 出版日期:2023-10-18 发布日期:2023-10-17
  • 通讯作者: 伍建榕，教授，博士生导师，研究方向为森林病理学及资源微生物利用，E-mail:1176279044@qq.com
  • 作者简介:杨娅琳，硕士，研究方向为森林病理学，E-mail:1319297640@qq.com
  • 基金资助:
    国家重点研发计划项目（2019YFD1002002）； 国家自然科学基金项目（31860208）

The relationship between arbuscular mycorrhizal fungi (AMF) and anthracnose occurrence in Camellia oleifera

YANG Yalin1, WU Ziqiang1, LIU Li1, ZHANG Donghua1, MA Huancheng2, WU Jianrong1   

  1. 1. Key Laboratory of Forest Disaster Early Warning and Control in Yunnan Universities, School of Biodiversity
    Protection, Southwest Forestry University, Kunming 650224, China; 2. Key Laboratory of Biodiversity Conservation
    in Southwest China, State Forestry Administration, School of Forestry, Southwest Forestry University,
    Kunming 650224, China
  • Online:2023-10-18 Published:2023-10-17

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摘要： 为应用丛枝菌根真菌（arbuscular mycorrhizal fungi, AMF）防控油茶炭疽病，探究土壤AMF与油茶炭疽病发生程度之间的关系。采用湿筛沉淀法和AMF定殖染色法探究油茶炭疽病发生样点土壤的AMF孢子密度、丰富度、多样性指数和菌根定殖率及病健植株发病率与病情指数间的关系。结果表明，病健油茶根部AMF孢子密度、定殖率具有显著差别，感病油茶土壤中AMF孢子的丰富度与多样性指数有下降趋势（P＜0.05）；AMF孢子密度、丰富度、定殖率与油茶炭疽病发病率和病情指数呈极显著负相关关系（P＜0.01），多样性指数与油茶炭疽病发病率和病情指数呈显著负相关关系（P＜0.05）。研究发现AMF孢子密度、菌根定殖率、丰富度和多样性指数越大，油茶炭疽病越轻，显示AMF对防治油茶炭疽病具有潜在应用价值。

关键词: 油茶炭疽病, 丛枝菌根真菌（AMF）, 真菌多样性, 丰富度指数, 生物防治

Abstract: In order to control Camellia oleifera anthracnose with arbuscular mycorrhizal fungi (AMF), the effects of AMF on Camellia oleifera anthracnose were investigated. The parameters such as AMF spore density, richness, diversity index, mycorrhizal colonization rate of C.oleifera and the disease occurrence were studied by wet screening precipitation and AMF colonization staining methods. Results showed that there were significant differences in AMF spore density and colonization rate between diseased and healthy C.oleifera roots. The spore density, richness and colonization rate of AMF were negatively correlated with the incidence rate and disease index of C.oleifera anthracnose, and the diversity index was also negatively correlated with the incidence rate and disease index of C.oleifera anthracnose. It showed that the higher the spore density, mycorrhizal colonization rate, richness and diversity index of AMF, the less the anthracnose of C.oleifera.

Key words: Camellia oleifera anthracnose, arbuscular mycorrhizal fungi (AMF), fungal diversity, richness index, biological control

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