生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (1): 60-.doi: 10.3969/j.issn.2095-1736.2023.01.060

• 研究报告 • 上一篇    下一篇

持续盐胁迫对沙漠小球藻生长的影响

李　红1,2, 谭　君2, 牟　云2, 高剑峰2   

  1. 1. 石河子大学 药学院 新疆植物药资源利用教育部重点实验室， 石河子 832003;
    2. 石河子大学 生命科学学院， 石河子 832003
  • 出版日期:2023-02-18 发布日期:2023-02-21
  • 通讯作者: 高剑峰，博士生导师，教授，研究方向为生物学，E-mail: jianfengg@shzu.edu.cn
  • 作者简介:李红，博士，副教授，研究方向为微藻生物技术，E-mail: 576918926@qq.com
  • 基金资助:
    国家自然科学基金项目（31460276）；石河子大学校级科研立项项目（ZZZC201840B）

Effect of sustained salt stress on the growth of desert Chlorella

LI Hong1,2, TAN Jun2, MU Yun2, GAO Jianfeng2   

  1. 1. Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, School of Pharmacy,
    Shihezi University, Shehezi 832003, China;
    2. College of Life Sciences, Shihezi University, Shihezi 832003, China
  • Online:2023-02-18 Published:2023-02-21

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摘要： 为探究不同盐胁迫水平下持续盐胁迫对TLD2A1、TLD6B、GTD7c-2和TGD8A1等4株沙漠小球藻生长的影响，研究不同NaCl添加量、不同胁迫时间下4株小球藻的OD680值、致死率、生物量、比生长速率的变化，筛选出对盐胁迫响应显著的小球藻，优化了其NaCl添加量的梯度。结果表明：除了添加0.2 mol/L NaCl胁迫下生物量增大外，随着NaCl添加量的增大，4株小球藻的OD680值、生物量、比生长速率受到的抑制程度均逐渐增大，致死率逐渐增大；随胁迫时间的延长，OD680值、致死率、生物量逐渐增大，比生长速率先增大后减小。总体而言，GTD7c-2耐盐，GTD8A1盐胁迫后生长不稳定，TLD2A1最不耐盐，TLD6B受盐胁迫的响应最明显。

关键词: 沙漠小球藻, 持续盐胁迫, 生长, 影响, 比较

Abstract: To explore the effects of continuous salt stress on the growth of four strains of desert Chlorellas TLD2A1, TLD6B, GTD7c-2 and TGD8A1 under different salt stress levels, the parameters of OD680, lethality, biomass, and specific growth rate of four strains of desert Chlorella were performed under different NaCl addition and different stress time.Chlorella with significant response to sustained salt stress was selected, and the gradient of NaCl addition was optimized. The results showed that, except for the increase in biomass under salt stress with the addition of 0.2 mol/L NaCl, the inhibition degree of OD680 value, biomass, and specific growth rate of the four Chlorella strains gradually increased and the lethal rate gradually increased as the increase of NaCl addition.With the extension of stress time, OD680 value, lethality and biomass gradually increased, and the specific growth rate first increased and then decreased.In general, GTD7c-2 was salt-tolerant, GTD8A1 grew unstable after salt stress, TLD2A1 was the least salt-tolerant, and TLD6B responsed most significantly to salt stress.

Key words: desert Chlorella, sustained salt stress, growth, influence, comparison

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